14 results on '"Santos, Tiago"'
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2. Current Challenges and Genomic Advances Towards the Development Resilient Coffee Genotypes to Abiotic Stresses
- Author
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dos Santos, Tiago Benedito, da Silva Ferreira, Marcia Flores, Marques, Isabel, Oliveira, Stefanie Cristina, Zaidan, Iasmine Ramos, Oliveira, Marcos Góes, Rodrigues, Weverton Pereira, Ribas, Alessandra Ferreira, Guyot, Romain, Ramalho, José Cochicho, Ribeiro-Barros, Ana I., Pereira, Luiz Filipe Protasio, Partelli, Fábio Luiz, and Kole, Chittaranjan, editor
- Published
- 2022
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3. An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses
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dos Santos, Tiago Benedito, Soares, João D. M., Lima, Joni E., Silva, Juliana C., Ivamoto, Suzana T., Baba, Viviane Y., Souza, Silvia G. H., Lorenzetti, Alan P. R., Paschoal, Alexandre R., Meda, Anderson R., Nishiyama Júnior, Milton Y., de Oliveira, Úrsula C., Mokochinski, João B., Guyot, Romain, Junqueira-de-Azevedo, Inácio L. M., Figueira, Antônio V. O., Mazzafera, Paulo, Júnior, Osvaldo R., Vieira, Luiz G. E., Pereira, Luiz F. P., and Domingues, Douglas S.
- Published
- 2019
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4. Nitrogen Starvation, Salt and Heat Stress in Coffee (Coffea arabica L.): Identification and Validation of New Genes for qPCR Normalization
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de Carvalho, Kenia, Bespalhok Filho, João Carlos, dos Santos, Tiago Benedito, de Souza, Silvia Graciele Hülse, Vieira, Luiz Gonzaga Esteves, Pereira, Luis Filipe Protasio, and Domingues, Douglas Silva
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- 2013
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5. Genome-Wide Identification, Evolution, and Expression Profile of Aquaporin Genes in Coffea canephora in Response to Water Deficit.
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Yaguinuma, Diliane Harumi, dos Santos, Tiago Benedito, de Souza, Silvia Graciele Hulse, Vieira, Luiz Gonzaga Esteves, and Ribas, Alessandra Ferreira
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GENE expression profiling , *DROUGHT tolerance , *COFFEE , *BASIC proteins , *GENES , *INSTANT coffee , *COFFEE beans - Abstract
Aquaporins (APQs) belong to the major intrinsic protein superfamily and play a key role in the transport of water and other solutes across cell membranes. Coffea canephora is an evergreen shrub used for making instant coffees. Genome analysis of C. canephora identified 33 putative aquaporin genes assigned to five subfamilies including seven plasma membrane intrinsic proteins (PIP), 9 tonoplast intrinsic proteins (TIP), 11 NOD26-like intrinsic proteins (NIP), 3 small basic intrinsic proteins (SIP), and 3 X intrinsic proteins (XIP). Generally, the AQPs gene structure was conserved within each subfamily, with exon numbers ranging from one to five. The prediction of the aromatic/arginine selectivity filter (ar/R) and Froger's positions indicated a noticeable difference in substrate specificity between subfamilies. Synteny analysis revealed high conservation of aquaporin genes in coffee. In silico expression analysis of the CcAQPs genes indicated that they were differentially expressed in various tissues. Members of CcPIPs and CcTIPs subfamilies were validated by real-time quantitative analysis in leaves of two genotypes of C. canephora with contrasting responses to water deficit (clone 14: drought-tolerant and clone 109A: drought-susceptible). Under severe water deficit, the relative expression of isoforms of both genes decreased in clone 14 compared with that under the irrigated condition, while clone 109A showed comparatively higher mRNA levels, with the exception of CcPIP1;2 in the stress condition. This study was the first to characterize and validate aquaporin genes in C. canephora in response to water deficit, and the findings may provide insights for biotechnological approaches to increase tolerance to drought. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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6. Caracterização nutricional de acessos provenientes da Etiópia de café arábica
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dos Santos, Tiago Benedito, Santos, Tiago Benedito, Meda, Anderson Rotter, Sitta, Renata Barrufaldi, Vespero, Eduardo Brandalize, Pavan, Marcos Antônio, Charmetant, Pierre, Carpentieri- Pípolo, Valéria, Pereira, Luiz Filipe Protasio, Vieira, Luiz Gonzaga Esteves, and Domingues, Douglas Silva
- Subjects
germoplasma ,fósforo ,germplasm ,Café ,phosphorous ,Coffee ,nitrogen ,nitrogênio - Abstract
Due to the increasing need for cultivars with high yield and low environmental impact, the introgression of traits related to nutrient utilization efficiency is an important strategy for coffee breeding. In this way, the characterization of accessions from the center of origin is an important tool for breeding programs to gain insight on relevant genotypes with potential interest for production of new cultivars. This work aims to characterize nutrient concentration in 12 Coffea arabica L. wild accessions from Ethiopia, in greenhouse conditions, in order to identify genotypes displaying differential accumulation of mineral nutrients in leaves, shoots and roots. Based on the established criteria, Ethiopian access E237/CAF032 showed differential accumulation for P in leaves and E565/CAF009, E386/CAF131 and E332/CAF023 displayed more accumulation of N, Zn and Mn in roots, respectively. This study identifies one accession with potential more P use efficiency and one accession with potential more N use efficiency, which is an important contribution to identify C. arabica wild genotypes with potential traits related to nutritional efficiency. Devido à necessidade cada vez maior de cultivares que apresentem boa produtividade com impactos menores ao meio ambiente, a introgressão de características relacionadas ao uso eficiente de nutrientes minerais é uma estratégia relevante para o melhoramento genético do café. Nesse sentido, a caracterização de acessos provenientes do centro de origem da espécie é um importante subsídio para os programas de melhoramento visando à seleção de materiais de interesse para produção de novas cultivares. Objetivou-se,no presente trabalho, caracterizar a concentração de nove nutrientes minerais em 12 acessos de Coffea arabica L., provenientes da Etiópia, em casa de vegetação, visando identificar genótipos com acumulação diferencial de nutrientes minerais em folhas, caule e raízes. Com base nos parâmetros estabelecidos, o acesso E237/CAF032 apresentou acúmulo diferencial para P, em folhas e E565/CAF009, E386/CAF131 e E332/CAF023 apresentaram concentração diferencial de N, Zn e Mn em raízes, respectivamente. O presente trabalho selecionou um acesso com potencial eficiência para uso de P e outro com potencial eficiência para o uso de N, o que pode contribuir em futuros estudos de seleção de materiais que possam ser utilizados na introgressão de características relacionadas à eficiência nutricional.
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- 2015
7. Transcriptional patterns of Coffea arabica L. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust.
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Yumi Baba, Viviane, Braghini, Masako Toma, dos Santos, Tiago Benedito, de Carvalho, Kenia, Moura Soares, João Danillo, Ivamoto-Suzuki, Suzana Tiemi, Maluf, Mirian P., Padilha, Lilian, Paccola-Meirelles, Luzia D., Pereira, Luiz Filipe, and Domingues, Douglas S.
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GLUTAMINE synthetase ,NITRATE reductase ,COFFEE ,CORROSION & anti-corrosives ,RUST diseases ,GENES ,STEEL corrosion - Abstract
This study evaluated the transcriptional profile of genes related to nitrogen (N) assimilation in coffee plants susceptible and resistant to rust fungi under N sufficiency and N suppression. For this purpose, we inoculated young coffee leaves with Hemileia vastatrix uredospores and collected them at 0, 12, 24 and 48 hours post-inoculation (HPI) to evaluate the relative expressions of genes encoding cytosolic glutamine synthetase (CaGS1), plastid glutamine synthetase (CaGS2), nitrate reductase (CaNR), and asparagine synthetase (CaAS). The genes exhibited distinct patterns of transcriptional modulation for the different genotypes and N nutritional regimes. The resistant genotype (I59) presented high levels of transcription in response to pathogen inoculation for CaNR and CaGS1 genes, evaluated under N sufficiency in the initial moments of infection (12 HPI). The gene CaGS1 also showed a peak at 48 HPI. The susceptible genotype (CV99) showed increased transcript rates of CaNR at 12 and 24 HPI in response to rust inoculation. The transcriptional patterns observed for CV99, under N suppression, were high levels for CaAS and CaGS
2 at all post-inoculation times in response to coffee leaf rust disease. In addition, CaGS1 was up-regulated at 48 HPI for CV99. Cultivar I59 showed high transcript levels at 12 HPI for CaAS and peaks at 24 and 48 HPI for CaGS2 in inoculated samples. Consequently, total chlorophyl concentration was influenced by N suppression and by rust infection. Regarding enzyme activities in vitro for glutamine synthetase and CaNR, there was an increase in infected coffee leaves (I59) and under N sufficiency. Moreover, CV99 was modulated in both N nutritional regimes for GS activity in response to rust. Our results indicate that N transport genes trigger a differential modulation between genotypes through the action of rust disease. [ABSTRACT FROM AUTHOR]- Published
- 2020
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8. IN SILICO ANALYSIS OF THE Dof TRANSCRIPTION FACTOR FAMILY IN Coffea canephora.
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Garcia, Vinícius, Ribas, Alessandra Ferreira, Vieira, Luiz Gonzaga Esteves, and dos Santos, Tiago Benedito
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TRANSCRIPTION factors ,COFFEE ,PLANT growth - Abstract
Copyright of Colloquium Agrariae is the property of Asociacao Prudentina de Educacao e Cultura (APEC) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2018
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9. Transcriptome Analysis of Leaves, Flowers and Fruits Perisperm of Coffea arabica L. Reveals the Differential Expression of Genes Involved in Raffinose Biosynthesis.
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Ivamoto, Suzana Tiemi, JúniorReis, Osvaldo, Domingues, Douglas Silva, dos Santos, Tiago Benedito, de Oliveira, Fernanda Freitas, Pot, David, Leroy, Thierry, Vieira, Luiz Gonzaga Esteves, Carazzolle, Marcelo Falsarella, Pereira, Gonçalo Amarante Guimarães, and Pereira, Luiz Filipe Protasio
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COFFEE ,GENETIC transcription in plants ,GENE expression in plants ,RAFFINOSE ,BIOSYNTHESIS - Abstract
Coffea arabica L. is an important crop in several developing countries. Despite its economic importance, minimal transcriptome data are available for fruit tissues, especially during fruit development where several compounds related to coffee quality are produced. To understand the molecular aspects related to coffee fruit and grain development, we report a large-scale transcriptome analysis of leaf, flower and perisperm fruit tissue development. Illumina sequencing yielded 41,881,572 high-quality filtered reads. De novo assembly generated 65,364 unigenes with an average length of 1,264 bp. A total of 24,548 unigenes were annotated as protein coding genes, including 12,560 full-length sequences. In the annotation process, we identified nine candidate genes related to the biosynthesis of raffinose family oligossacarides (RFOs). These sugars confer osmoprotection and are accumulated during initial fruit development. Four genes from this pathway had their transcriptional pattern validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, we identified ~24,000 putative target sites for microRNAs (miRNAs) and 134 putative transcriptionally active transposable elements (TE) sequences in our dataset. This C. arabica transcriptomic atlas provides an important step for identifying candidate genes related to several coffee metabolic pathways, especially those related to fruit chemical composition and therefore beverage quality. Our results are the starting point for enhancing our knowledge about the coffee genes that are transcribed during the flowering and initial fruit development stages. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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10. Salt stress alters the cell wall polysaccharides and anatomy of coffee (Coffea arabica L.) leaf cells.
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de Lima, Rogério Barbosa, dos Santos, Tiago Benedito, Esteves Vieira, Luiz Gonzaga, Lúcio Ferrarese, Maria de Lourdes, Ferrarese-Filho, Osvaldo, Donatti, Lucélia, Torres Boeger, Maria Regina, and de Oliveira Petkowicz, Carmen Lúcia
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COFFEE , *LEAVES , *POLYSACCHARIDES , *PLANT anatomy , *ELECTRON microscopy , *PLANT cell walls - Abstract
Coffea arabicais the most important agricultural commodity in the world, and salinity is a major threat to its sustainable irrigation. Coffee leaf polysaccharides from plants subjected to salt stress were extracted and the leaves visualized through optical and electron microscopy. Alterations were detected in the monosaccharide composition of the pectin and hemicelluloses, with increases in uronic acid in all fractions. Changes in the polysaccharides were confirmed by HPSEC and FTIR. Moreover, the monolignol content was increased in the final residue, which suggests increased lignin content. The cytoplasm was altered, and the chloroplasts appeared irregular in shape. The arrangement of the stroma lamellae was disordered, and no starch granules were present. It was concluded that leaves ofC. arabicaunder salt stress showed alterations in cell wall polysaccharides, increased monolignol content and structural damage to the cells of the mesophyll. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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11. Heat stress causes alterations in the cell-wall polymers and anatomy of coffee leaves (Coffea arabica L.)
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Lima, Rogério Barbosa, dos Santos, Tiago Benedito, Vieira, Luiz Gonzaga Esteves, Ferrarese, Maria de Lourdes Lúcio, Ferrarese-Filho, Osvaldo, Donatti, Lucélia, Boeger, Maria Regina Torres, and Petkowicz, Carmen Lúcia de Oliveira
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PHYSIOLOGICAL effects of heat , *POLYMERS , *PLANT anatomy , *COMPOSITION of leaves , *COFFEE , *COMPARATIVE studies , *PLANT cells & tissues - Abstract
Abstract: Coffee plants were subjected to heat stress (37°C) and compared with control plants (24°C). Cell wall polysaccharides were extracted using water (W), EDTA (E) and 4M NaOH (H30 and H70). In addition, monolignols were analyzed, and the leaves were observed by microscopy. Plants under heat stress accumulated higher contents of arabinose and galactose in fraction W. Xylose contents were observed to decrease in H30 fractions after the heat stress, whereas galactose and uronic acid increased. H70 fractions from plants exposed to heat stress showed increased xylose contents, whereas the contents of arabinose and glucose decreased. Differences in the molar-mass profiles of polysaccharides were also observed. The primary monolignol contents increased after the heat stress. Structural alterations in palisade cells and ultrastructural damage in chloroplasts were also observed. Our results demonstrate that the chemical profile of coffee cell-wall polymers and structural cell anatomy change under heat stress. [Copyright &y& Elsevier]
- Published
- 2013
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12. Nitrogen Starvation, Salt and Heat Stress in Coffee ( Coffea arabica L.): Identification and Validation of New Genes for qPCR Normalization.
- Author
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Carvalho, Kenia, Bespalhok Filho, João, Santos, Tiago, Souza, Silvia, Vieira, Luiz, Pereira, Luis, and Domingues, Douglas
- Abstract
Abiotic stresses are among the most important factors that affect food production. One important step to face these environmental challenges is the transcriptional modulation. Quantitative real-time PCR is a rapid, sensitive, and reliable method for the detection of mRNAs and it has become a powerful tool to mitigate plant stress tolerance; however, suitable reference genes are required for data normalization. Reference genes for coffee plants during nitrogen starvation, salinity and heat stress have not yet been reported. We evaluated the expression stability of ten candidate reference genes using geNorm PLUS, NormFinder, and BestKeeper softwares, in plants submitted to nitrogen starvation, salt and heat stress. EF1, EF1α, GAPDH, MDH, and UBQ10 were ranked as the most stable genes in all stresses and software analyses, while RPL39 and RPII were classified as the less reliable references. For reference gene validation, the transcriptional pattern of a Coffea non-symbiotic hemoglobin ( CaHb1) was analyzed using the two new recommended and the most unstable gene references for normalization. The most unstable gene may lead to incorrect interpretation of CaHb1 transcriptional analysis. Here, we recommend two new reference genes in Coffea for use in data normalization in abiotic stresses: MDH and EF1. [ABSTRACT FROM AUTHOR]
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- 2013
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13. FISH using a gag-like fragment probe reveals a common Ty3- gypsy-like retrotransposon in genome of Coffea species.
- Author
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Yuyama, Priscila Mary, Pereira, Luiz Filipe Protasio, dos Santos, Tiago Benedito, Sera, Tumoru, Vilas-Boas, Laurival Antonio, Lopes, Fabrício Ramon, Carareto, Claudia Marcia Aparecida, Vanzela, André Luís Laforga, and Puertas, M.
- Subjects
RETROTRANSPOSONS ,COFFEE ,PLANT genomes ,KARYOTYPES ,POLYMERASE chain reaction - Abstract
Copyright of Genome is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2012
- Full Text
- View/download PDF
14. Expression of three galactinol synthase isoforms in Coffea arabica L. and accumulation of raffinose and stachyose in response to abiotic stresses
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dos Santos, Tiago B., Budzinski, Ilara G.F., Marur, Celso J., Petkowicz, Carmen L.O., Pereira, Luiz F.P., and Vieira, Luiz G.E.
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GENE expression , *COFFEE , *RAFFINOSE , *OLIGOSACCHARIDES , *TRANSCRIPTION factors , *REACTIVE oxygen species , *ENDOSPERM , *GLYCOSYLTRANSFERASES , *PLANT growth - Abstract
Abstract: Galactinol synthase (EC 2.4.1.123; GolS) catalyzes the first step in the synthesis of raffinose family oligosaccharides (RFOs). Their accumulation in response to abiotic stresses implies a role for RFOs in stress adaptation. In this study, the expression patterns of three isoforms of galactinol synthase (CaGolS1-2-3) from Coffea arabica were evaluated in response to water deficit, salinity and heat stress. All CaGolS isoforms were highly expressed in leaves while little to no expression were detected in flower buds, flowers, plagiotropic shoots, roots, endosperm and pericarp of mature fruits. Transcriptional analysis indicated that the genes were differentially regulated under water deficit, high salt and heat stress. CaGolS1 isoform is constitutively expressed in plants under normal growth conditions and was the most responsive during all stress treatments. CaGolS2 is unique among the three isoforms in that it was detected only under severe water deficit and salt stresses. CaGolS3 was primarily expressed under moderate and severe drought. This isoform was induced only at the third day of heat and under high salt stress. The increase in GolS transcription was not reflected into the amount of galactinol in coffee leaves, as specific glycosyltransferases most likely used galactinol to transfer galactose units to higher homologous oligosaccharides, as suggested by the increase of raffinose and stachyose during the stresses. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
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