1. Fabrication and characterization of a novel collagen-catechol hydrogel.
- Author
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Duan L, Yuan Q, Xiang H, Yang X, Liu L, and Li J
- Subjects
- Animals, Benzaldehydes chemistry, Cattle, Cross-Linking Reagents chemistry, Elastic Modulus, Mice, NIH 3T3 Cells, Polymerization, Temperature, Biocompatible Materials chemistry, Catechols chemistry, Collagen chemistry, Hydrogels chemistry
- Abstract
3,4-Dihydroxybenzaldehyde, a derivative of catechol and an agent with an extensive pharmacological and biological activities, was used to modify collagen and prepared hydrogels. The aldehyde group of 3,4-dihydroxybenzaldehyde interacted with the ɛ-amino group of collagen, and then the catechol group of 3,4-dihydroxybenzaldehyde was oxidized and self-polymerized. The chemical network formed due to the cross-linking bridges of polymerized catechol groups among collagen molecules, resulting in the transformation from solution to hydrogel. The results of the Fourier-transform infrared measurement indicated that the triple helix structure of collagen was integrated after cross-linking. The appearance of hydrogels changed from golden to dark brown with the increasing 3,4-dihydroxybenzaldehyde dose. When the weight ratio of 3,4-dihydroxybenzaldehyde and collagen increased from 0 to 2:1, the thermal denaturation temperature of collagen increased from 40.2 to 77.6℃ while the elastic modulus of collagen increased from 13.6 to 1061.4 Pa. The addition of 3,4-dihydroxybenzaldehyde also caused more compact morphologies and a dramatic enhancement in the enzymatic resistance of hydrogels. Moreover, the results of cell proliferation assay demonstrated the favorable biocompatibility of collagen hydrogels with 3,4-dihydroxybenzaldehyde. These promising data indicate that the novel hydrogels had significant potential for applications.
- Published
- 2018
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