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Safendra Siregar, Ricky Adriansjah, Jupiter Sibarani, Akhmad Mustafa Department of Urology, Faculty of Medicine, Universitas Padjadjaran, General Hospital Hasan Sadikin, Bandung, IndonesiaCorrespondence: Safendra SiregarDepartment of Urology, Faculty of Medicine, Universitas Padjadjaran, General Hospital Hasan Sadikin Bandung, 16 Jalan Makmur, Bandung, IndonesiaTel +62 811 227 180Email safendra.sir@yahoo.co.idAkhmad MustafaDepartment of Urology, Faculty of Medicine, Universitas Padjadjaran, General Hospital Hasan Sadikin Bandung, 28A Jalan Cikutra Baru Raya, Bandung 40124, IndonesiaTel +62 811 232 827Email mustafa.urologi@gmail.comIntroduction: The ischemic process in priapism can lead to displacement of normal tissue with fibrotic tissue, due to collagen deposition, and eventually leads to erectile dysfunction. Many studies have identified that the supernatant of adipose tissue–derived stem cells (ADSCs) significantly ameliorates fibrosis of different tissue, but limited attention has been paid to its efficacy on fibrosis of the corpora cavernosa.Methods: A total of 22 Wistar rats divided into five groups, with two groups each consisting of five male wistar rats with priapism without human ADSC (hADSC) therapy (group I) and two other groups consisting of five rats with priapism, were given 10 6 cells’ intracorporeal hADSC injection after 12 hours of penile clamping (group II) were euthanized after 2 and 4 weeks of observation. The last group consisted of two rats without any treatment or model (group III). Following euthanasia, penises were harvested for TGFβ 1 and collagen type I measurement using ELISA. Statistical analysis using independent-sample t-tests was done with SPSS 21.0.Results: Penile TGFβ 1 concentration in the treatment group was significantly lower in the second and fourth weeks of observation (p 2=0.004, p 4=0.003), and collagen type I was significantly lower in the second and fourth weeks (p 2=0.003, p 4=0.011).Conclusion: Intracorporeal hADSC injection limited the fibrosis process in a priapism model. Although the mechanism was unclear, it may be related to the potential of hADSCs to produce various growth factors that could limit TGFβ 1 and collagen production.Keywords: human adipose–derived stem cell, collagen type I, priapism, TGFβ 1