1. Characterization of gold nanoparticles produced by biogenic synthesis using Serratia marcescens NBL1001
- Author
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L L Ilag, Lilia M. Fernando, Z. K. Baybay, Andrew D. Montecillo, L C Villegas, and R M Mendoza
- Subjects
Absorbance ,medicine.diagnostic_test ,biology ,Colloidal gold ,Scanning electron microscope ,Chemistry ,Spectrophotometry ,Serratia marcescens ,medicine ,Spectroscopy ,biology.organism_classification ,Nuclear chemistry - Abstract
The wide applications of gold nanoparticles in fields such as electronics and biomedicine (targeted-drug delivery) have caught the interest of many researchers. In this study, Serratia marcescens NBL 1001 was screened for its ability to synthesize gold nanoparticles (AuNP). Colorimetric change from light yellow to purple, 24 h after the supplementation of 1mM tetrachloroaric acid (HAuCl4), indicated production of AuNP. UV-Vis scanning spectrophotometry of bacterial suspension, cell-free supernatant and reconstituted cell pellets of S. marcescens NBL1001 showed absorbance peaks characteristic of AuNP (500-600 nm) at 554 nm, 556 nm and 550 nm respectively. Gold nanoparticles with sizes ranging from 11.78 to 46. 03 nm with mean size of 25.28 nm (n = 30) were observed under the scanning electron microscope and the presence of elemental gold (Au0) was confirmed by energy dispersive X-ray spectroscopy.
- Published
- 2019