1. Self-assembly of DNA nanoparticles through multiple catalyzed hairpin assembly for enzyme-free nucleic acid amplified detection.
- Author
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He H, Dai J, Meng Y, Duan Z, Zhou C, Zheng B, Du J, Guo Y, and Xiao D
- Subjects
- Base Pairing, Biocatalysis, Catalysis, DNA analysis, Limit of Detection, Microscopy, Atomic Force, Nanoparticles ultrastructure, Nucleic Acid Conformation, Biosensing Techniques methods, Colorimetry methods, DNA chemistry, Nanoparticles chemistry, Nucleic Acid Amplification Techniques, Silver chemistry
- Abstract
It is known that DNA molecules can be used to build a various of complicated geometrical DNA nanostructures with programmable sequence design, and these DNA nanomaterials show a promising application in biotechnology and biomedicine. However, the construction of large-sized three dimensional DNA-based nanomaterials still remains a challenge. In this work, we propose a new strategy that only employs one target DNA to trigger multiple catalyzed hairpin assembly (CHA) reactions and sticky ends self-assembly to prepare hundreds of nanometer-sized DNA nanoparticles. Moreover, the obtained DNA nanoparticles can be served as efficient biosensors for sensitive colorimetric nucleic acids detection with a detection limit of 7.7pM., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2018
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