1. Effect of electroacupuncture pretreatment on the protein expression of c-fos in fastigial nucleus and lateral hypothalamus area in rats with acute myocardial ischemia-reperfusion injury
- Author
-
Wei Xiao-tong, Shao Xue-fang, Yu Qing, Zhang Ya-ting, Cai Rong-lin, and Hu Ling
- Subjects
Cardiac function curve ,medicine.medical_specialty ,genetic structures ,Lateral hypothalamus ,Electroacupuncture ,medicine.medical_treatment ,0211 other engineering and technologies ,02 engineering and technology ,c-Fos ,Lesion ,03 medical and health sciences ,fluids and secretions ,0302 clinical medicine ,Internal medicine ,021105 building & construction ,medicine ,Acupuncture ,Fastigial nucleus ,biology ,business.industry ,eye diseases ,030205 complementary & alternative medicine ,Meridian (perimetry, visual field) ,Endocrinology ,Complementary and alternative medicine ,biology.protein ,medicine.symptom ,business - Abstract
To observe the effect of electroacupuncture (EA) pretreatment on the protein expression of c-fos in fastigial nucleus (FN) and lateral hypothalamus area (LHA) in rats with acute myocardial ischemia-reperfusion injury (MIRI), and to explore the role and mechanism of FN and LHA in EA at the Heart Meridian fighting against acute MIRI reaction. Seventy Sprague-Dawley rats were randomly divided into a sham operation group, a model group, an EA-Heart Meridian group and an EA-Lung Meridian group, with 14 rats in each group; an LHA lesion plus EA-Heart Meridian group (LHA+EA-Heart Meridian group) and a FN lesion plus EA-Heart Meridian group (FN+EA-Heart Meridian group), with 7 rats in each group. Except the sham operation group, the left anterior descending branch of coronary artery was ligated to establish acute MIRI rat models in the other 5 groups. In the three groups with EA-Heart Meridian treatment, Shenmen (HT 7) and Tongli (HT 5) were selected; Taiyuan (LU 9) and Lieque (LU 7) were selected in the EA-Lung Meridian group. All the EA groups received EA stimulation prior to modeling, with 1 mA in current intensity and 2 Hz in frequency, 20 min each time, once a day for a total of 7 d. The sham operation group and the model group did not receive EA stimulation. The electrocardiogram was observed in the rats to analyze the ST-segment deviation and cardiac arrhythmia score. The expression of c-fos protein in FN and LHA was detected by immunohistochemistry method. Compared with the sham operation group, the ST-segment deviation, cardiac arrhythmia score and the expression of c-fos protein in the FN and LHA increased significantly in the model group (all P
- Published
- 2021