1. The biological mechanism of a lower carbon/nitrogen ratio increases methane emissions during vegetable waste composting.
- Author
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Chen Y, Xu J, Liu B, Zhang Z, and Xu M
- Subjects
- Air Pollutants analysis, Methane metabolism, Methane analysis, Carbon metabolism, Carbon analysis, Composting, Nitrogen analysis, Nitrogen metabolism, Vegetables
- Abstract
The initial carbon/nitrogen (C/N) ratio is one of the most important factors impacting composting processes, such as methane (CH
4 ) emissions. However, the effects of the C/N ratio on CH4 emissions and the associated biological mechanisms during vegetable waste composting are largely unknown. In this study, a lab-scale experiment was conducted to investigate the effects of different C/N ratios on CH4 emissions and the mechanisms associated with methane-metabolizing microorganisms (methanogens and methanotrophs) during capsicum straw composting. The initial C/N ratios were set to 18, 30 and 50 to simulate the low (L), medium (M) and high (H) C/N ratios, respectively. The results showed that CH4 emissions were mainly concentrated in the thermophilic phase and that the cumulative CH4 emissions were significantly greater in the L treatment than in the M and H treatments by 10.8 and 15.4 times, respectively. During the methanogenic process, the relative abundance of the dominant genus Methanoculleus (47.59 % ∼ 76.92 %) was higher than in the L treatment than in the M and H treatments at the thermophilic and maturation stages, and the Chao1 index and the mcrA gene abundance followed the order of L > M > H at each composting stage. During the methanotrophic process, the dominant genus unclassified_d_bacteria (51.3 % ∼ 91.87 %), Chao1 index, pmoA gene abundance and CO2 emissions were in the order of L > M > H at each composting stage. This pattern suggests that a lower C/N ratio simultaneously enhanced CH4 production and oxidation. A structural equation model further revealed that the methanogenic community, which was driven directly by the relative contents of hemicellulose and cellulose in the substrates, as indicated by the C/N ratio, made greater contributions to CH4 emissions than did the methanotrophic community. In conclusion, a lower C/N ratio increased CH4 emissions mainly by regulating the population and composition of methanogen community., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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