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The recalcitrance of green waste, caused by its high lignocellulose content, is a technical challenge for accelerating green waste composting. However, because lignocellulose degradation in litter (similar to green waste) can be promoted during the freeze-thaw season, and the composting is difficult to implement in this period (due to the low temperature); seasonal freeze-thaw was intended to be used as a pretreatment strategy for the existing technical challenge in the winter of cold regions. In this process, green waste was pretreated with seasonal freeze-thaw to enhance its lignocellulose degradation for subsequent composting. To verify this assumption, two strategies for the pretreatment were used: the green waste was either drenched or immersed in water during the freeze-thaw season, and the effects on subsequent composting were evaluated. The results demonstrated that both strategies can significantly promote the mineralization of TOC (total organic carbon, by 2.73%-8.01% compared with the control, the following comparisons were all based on the control), TN (total nitrogen, by 0.21%-0.52%), and lignocellulose (lignin degradation was promoted by 3.52%-3.73%, cellulose degradation was promoted by 13.23%-14.26%) during composting and that the synthesis of humus was also enhanced (by 19.19%-21.43%). Furthermore, since the loss of NH 4 + N and NO 3 - N was significantly less in the drenched treatment than in the immersed treatment (by 9.15% for the loss of NH 4 + N and 7.66% for the loss of NO 3 - N), drenching the green waste during the freeze-thaw season might be a better strategy than immersing for nitrogen conservation. An additional advantage of drenching compared to immersing is water conservation., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

This research was conducted to compare chemical and microbiological properties during aerobic composting (AC) and vermicomposting (VC) of green waste. Relative to AC, VC significantly decreased the pH and lignin and cellulose contents, and significantly increased the electrical conductivity and total N and available P contents. For AC, BIrii41_norank (order Myxococcales) was the major bacterial genus at 30 d and again became dominant genus from 90-150 d, with relative abundances of 2.88% and 4.77-5.19%, respectively; at 45 d and 60 d, the dominant bacterial genus was Nitrosomonadaceae_uncultured (order Nitrosomonadales) with relative abundances of 2.83-7.17%. For VC, the dominant bacterial genus was BIrii41_norank (except at 45 d), which accounted for 2.11-7.96% of the total reads. The dominant fungal class was Sordariomycetes in AC (relative abundances 39.2-80.6%) and VC (relative abundances 42.1-69.5%). The abundances of microbial taxa and therefore the bacterial and fungal community structures differed between VC and AC. The quality of the green waste compost product was higher with VC than with AC. These results will also help to achieve further composting technology breakthroughs in reducing the composting time and improving compost quality., Competing Interests: The authors have declared that no competing interests exist.

The aims of this study are to determine the feasibility of applying QROD (quadratic regression orthogonal design) to optimize a combination of microorganisms and to develop a composite inoculum for promoting lignocellulose degradation during GWC (green waste composting). This feasibility was studied in a laboratory scale experiment, using three lignocellulolytic microorganisms, isolated from the mature phase of GWC by the dilution plating method. After the feasibility was confirmed, a composite inoculum was developed through the results of the optimization, whose effect was evaluated by comparing it with Phanerochaete chrysosporium and EM (Effective Microorganisms) in a pilot scale experiment of GWC. The use of QROD to finish this optimization was proven feasible, because the p value of the regression equation was less than 0.05 (0.0108), meaning that the quadratic regression model is suitable for describing the relationship between the combination of the three microorganisms and their ability to degrade lignocellulose. Additional proof of this feasibility is that the composite inoculum in the quadratic regression orthogonal experiment demonstrated lignocellulose degradation ability similar to the GWC experiment. Although the lignin degradation ability of the composite inoculum did not surpass Phanerochaete chrysosporium, it was stronger than EM. Meanwhile, cellulose degradation ability and humus synthesis ability of the composite inoculum were stronger than for Phanerochaete chrysosporium and were close to EM. It is hard to tell which inoculum is the best since each inoculum had advantages in different aspects, while the composite inoculum still showed a considerable effect of lignocellulose degradation during GWC., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Abstract Managing municipal green waste is a challenge to municipalities, partly because of the slow rate of decomposition of green waste during composting due to its high lignin and cellulose contents. Hence, this study evaluated the effect of alkyl polyglycoside (APG), a biosurfactant, and the earthworm Eisenia fetida on the composting process. Addition of APG and E. fetida significantly increased total bacteria, cellulolytic fungi, phosphate solubilizing bacteria and nitrogen fixing bacteria populations, and the activities of cellulase, urease and alkaline phosphatase in composts as compared with the control. The APG and earthworm treatments also increased surface roughness and porosity of the green waste; Compared with control, APG and earthworm addition increased the degradation rate of TOC, lignin and cellulose by 5.9–17.9, 10.3–32.0 and 10.8–18.8%, respectively, and resulted in better compost quality, as was reflected in the neutral pH, higher cation exchange capacity (CEC) and nutrient concentrations (N, P, K, Ca, Mg, Fe, Cu, Zn, Mn). Final germination percentage and growth rate of tomato, eggplant and pepper seedlings were higher (P < 0.05) or similar in all composts produced with the addition of APG and earthworm, while plant growth was lower (P < 0.05) in the compost produced with the control than in peat substrate. The combination of APG+ E. fetida enhanced the decomposition of green waste and improved final compost quality the most. Further research is needed to determine the best level of APG addition and optimum earthworm density for composting green waste. Highlights • Alkyl polyglycoside (APG) and/or E. fetida addition enhanced lignin degradation. • APG and/or E. fetida addition increased disaggregation of green waste. • APG and/or fetida addition improved nutrient availability in compost. • The compost supports the growth of tomato, eggplant and pepper seedlings. [ABSTRACT FROM AUTHOR]