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Start Over Author "Hagemeier Sebastian" Topic confocal microscopy
3 results on '"Hagemeier Sebastian"'

1. Microsphere-assistance in microscopic and confocal imaging.

Author

Hüser, Lucie, Hagemeier, Sebastian, Pahl, Tobias, and Lehmann, Peter

Subjects
*MICROSPHERES, *MICROSCOPY, *CONFOCAL microscopy, *X-ray diffraction, *RADIOPACITY
Abstract

Topographical as well as microscopic imaging of nanoscale surfaces plays a pivotal role across various disciplines. Nevertheless, achieving fast, label-free, and accurate characterization of laterally expanded structures below the diffraction limit remains challenging. Recent studies highlight the use of microsphere assistance for resolution improvement. Confocal microscopy, augmented by microspheres, enables the imaging of small structures that were previously inaccessible. This is experimentally compared with microsphere-assisted microscopy (MAM) to underline the decisive role of the confocal effect. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Modeling microcylinder-assisted conventional, interference and confocal microscopy.

Author

Pahl, Tobias, Hüser, Lucie, Eckhardt, Tim, Hagemeier, Sebastian, Rosenthal, Felix, Diehl, Michael, and Lehmann, Peter

Subjects
MICROSPHERES, CONFOCAL microscopy, SURFACE topography, FINITE element method, DETECTORS
Abstract

We present how to develop virtual microcylinder- or microsphere-assisted surface topography measurement instruments. As the most critical part, the interaction between light, microcylinder and measurement object is considered based on the finite element method (FEM). Results are obtained for microcylinder-assisted conventional, interference, and confocal microscopes without necessity to repeat the time-consuming FEM simulations for each sensor. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Rigorous 3D modeling of confocal microscopy on 2D surface topographies.

Author

Pahl, Tobias, Hagemeier, Sebastian, Bischoff, Jörg, Manske, Eberhard, and Lehmann, Peter

Subjects
SURFACE topography, CONFOCAL microscopy, FOURIER transform optics, FINITE element method, APODIZATION
Abstract

Although optical 3D topography measurement instruments are widespread, measured profiles suffer from systematic deviations occurring due to the wave characteristics of light. These deviations can be analyzed by numerical simulations. We present a 3D modeling of the image formation of confocal microscopes. For this, the light-surface interaction is simulated using two different rigorous methods, the finite element method and the rigorous coupled-wave analysis. The image formation in the confocal microscope is simulated using a Fourier optics approach. The model provides high accuracy and advantages with respect to the computational effort as a full 3D model is applied to 2D structures and the lateral scanning process of the confocal microscope is considered without repeating the time consuming rigorous simulation of the scattering process. The accuracy of the model is proved considering different deterministic surface structures, which usually cause strong systematic deviations in measurement results. Further, the influences of apodization and a finite pinhole size are demonstrated. [ABSTRACT FROM AUTHOR]

Published
2021
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