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According to the characteristics of the reverse osmosis concentrate (ROC) generated from Iron and Steel Company, we used three sets of parallel horizontal subsurface flow (HSF) constructed wetlands with different plants and substrate layouts to treat the high salinty wastewater. The plant growth and removal efficiencies under saline condition were evaluated. The evaluation was based entirely on routinely-collected water quality data, physical and chemical characteristics of the plants (Phragmites australis, Typha latifolia, Iris wilsonii, and Scirpus planiculmis). The principal parameters of concern in the effluent were chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP). The results showed that the constructed wetlands were able to remove COD, TN and TP from ROC. S. planiculmis was not suitable for treatment of high saline wastewater. The amount of metals accumulated in constructed wetland plants was sequencing as K>Ca>Na>Mg>Zn>Cu. More than 70% of metals were accumulated in the aboveground of Phragmites australis. The constructed wetland filled with gravel and manganese ore and planted with Phragmites australis, Typha latifolia had the best performance of pollutant removal, with average removal of 49.96%, 39.45% and 72.01% for COD, TN and TP, respectively. The effluent water quality met the regulation in China. These results suggested HSF constructed wetland planted with Phragmites australis and Typha latifolia can be applied in ROC pollutants removal.