1. An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA
- Author
-
Hafizullah Noori, François Poumarat, Karim Tounkara, Isabel Nkando, François Thiaucourt, Lucia Manso-Silvan, Willy Schauwers, Martha Yami, Deodass Meenowa, Nadia Mukhtar, Ghulam Mohammad Ziay, Hezron Wesonga, Florence Tardy, Karomatullo Hamroev, Armelle Peyraud, Mohibullah Halimi, Mahmad Reshad Jaumally, Charles Bodjo, Stéphane Ostrowski, Mullojon Amirbekov, Shiferaw Jenberie, Ali Madad Rajabi, Eric Cardinale, Tahir Yaqub, Muhammad Zubair Shabbir, Tillo Tilloev, Contrôle des maladies animales exotiques et émergentes (UMR CMAEE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Mycoplasmoses des Ruminants - UMR (MYCO), Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire de Lyon [ANSES], Kenya Agricultural Research Institute, Centre de Recherche et de Veille sur les Maladies Émergentes dans l'Océan Indien (CRVOI), Université de La Réunion (UR), European Union [DCI-FOOD/ 2009/226-469], United States Department of State through the American Association for the Advancement of Science (AAAS), [S-LMAQM-09-GR-055], Laboratoire de Lyon, Contrôle des maladies animales exotiques et émergentes [Montpellier] ( CMAEE ), Institut National de la Recherche Agronomique ( INRA ) -Centre de coopération internationale en recherche agronomique pour le développement [CIRAD] : UMR15, Mycoplasmoses des ruminants [Lyon], Université de Lyon-VetAgro Sup ( VAS ), VetAgro Sup ( VAS ), ANSES, Centre de Recherche et de Veille sur les Maladies Émergentes dans l'Océan Indien ( CRVOI ), and Université de la Réunion ( UR )
- Subjects
Tajikistan ,Veterinary medicine ,vaccin ,Internationality ,Seroprevalence ,séroprévalence ,L73 - Maladies des animaux ,Global Health ,Serology ,Mycoplasma capricolum ,Contagious caprine pleuropneumonia ,0403 veterinary science ,Contagious ,maurice ,Seroepidemiologic Studies ,Monoclonal ,Medicine ,Pakistan ,0303 health sciences ,Goat Diseases ,Pleuropneumonia ,biology ,Goats ,Antibodies, Monoclonal ,Vaccine quality control ,04 agricultural and veterinary sciences ,General Medicine ,Complement fixation test ,3. Good health ,Competitive ELISA ,Bacterial vaccine ,Bacterial Vaccines ,Mauritius ,Research Article ,040301 veterinary sciences ,Kenya ,Ethiopia ,Afghanistan ,Enzyme-Linked Immunosorbent Assay ,Antibodies ,03 medical and health sciences ,Animals ,Pleuropneumonia, Contagious ,030304 developmental biology ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,General Veterinary ,[ SDV ] Life Sciences [q-bio] ,business.industry ,medicine.disease ,biology.organism_classification ,veterinary(all) ,Virology ,ethiopie ,business ,tadjikistan - Abstract
Background Few serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae, the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, the test prescribed for international trade purposes, uses a crude antigen that cross-reacts with all the other mycoplasma species of the “mycoides cluster” frequently infecting goat herds. The lack of a more specific test has been a real obstacle to the evaluation of the prevalence and economic impact of CCPP worldwide. A new competitive ELISA kit for CCPP, based on a previous blocking ELISA, was formatted at CIRAD and used to evaluate the prevalence of CCPP in some regions of Kenya, Ethiopia, Mauritius, Tajikistan and Pakistan in an international collaborative study. Results The strict specificity of the test was confirmed in CCPP-free goat herds exposed to other mycoplasma species of the “mycoides cluster”. Prevalence studies were performed across the enzootic range of the disease in Africa and Asia. Seroprevalence was estimated at 14.6% in the Afar region of Ethiopia, whereas all the herds presented for CCPP vaccination in Kenya tested positive (individual seroprevalence varied from 6 to 90% within each herd). In Mauritius, where CCPP emerged in 2009, nine of 62 herds tested positive. In Central Asia, where the disease was confirmed only recently, no positive animals were detected in the Wakhan District of Afghanistan or across the border in neighboring areas of Tajikistan, whereas seroprevalence varied between 2.7% and 44.2% in the other districts investigated and in northern Pakistan. The test was also used to monitor seroconversion in vaccinated animals. Conclusions This newly formatted CCPP cELISA kit has retained the high specificity of the original kit. It can therefore be used to evaluate the prevalence of CCPP in countries or regions without vaccination programs. It could also be used to monitor the efficacy of vaccination campaigns as high-quality vaccines induce high rates of seroconversion.
- Published
- 2014
- Full Text
- View/download PDF