1. Dextran gadolinium complex containing folate groups as a potential magnetic resonance imaging contrast agent.
- Author
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Du, Hui-juan, Shen, Yan-chun, Liu, Yu-ping, Han, Lin, Zheng, Yao, Yan, Guo-ping, Tu, Yuan-yuan, Wu, Jiang-yu, Guo, Qing-zhong, Zhang, Yun-fei, Xia, Xiao-tian, Lan, Xiao-li, and Zhang, Yong-xue
- Subjects
DEXTRAN ,GADOLINIUM ,METAL complexes ,FOLIC acid ,MAGNETIC resonance imaging ,CONTRAST media - Abstract
Folate-containing dextran ligand (FA-Dextran-DTPA) was synthesized by the incorporation of diethylenetriaminepentaacetic acid (DTPA) and folate (FA) as a tumor-targeting group into dextran as a polymer carrier. This ligand was further reacted with gadolinium chloride to make a dextran gadolinium complex FA-Dextran-DTPA-Gd. The ligand and its gadolinium complex were characterized by H-NMR, FTIR, UV-Vis, average particle sizes and zeta potential, as well. In vitro properties including relaxivity, cytotoxicity assay, cellular uptake assay, and magnetic resonance imaging (MRI) were also evaluated. Compared with Gd-DTPA, FA-Dextran-DTPA-Gd possessed obviously higher relaxation effectiveness and lower cytotoxicity to HeLa cells. FA-Dextran-DTPA-Gd had a high affinity to the H460 and MDA-MB-231 tumor cells and can be taken up selectively by these tumor cells. Moreover, FA-Dextran-DTPA-Gd showed enhanced signal intensities (SI) of MRI and enhanced the contrast of MR images of tumor cells. These results indicated that FA-Dextran-DTPA-Gd showed the potential as a tumor-targeting contrast agent in MRI. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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