1. Highly Stable Docetaxel-Loaded Nanoparticles Based on Poly(D,L-lactide)- b -Poly(ethylene glycol) for Cancer Treatment: Preparation, Characterization, and In Vitro Cytotoxicity Studies.
- Author
-
Kuznetsova, Ekaterina V., Sedush, Nikita G., Puchkova, Yulia A., Aleshin, Sergei V., Yastremsky, Evgeny V., Nazarov, Alexey A., and Chvalun, Sergei N.
- Subjects
DOCETAXEL ,ETHYLENE glycol ,CANCER treatment ,NANOPARTICLES ,COPOLYMERS ,BLOCK copolymers - Abstract
Stability and narrow size distribution are among the main requirements that apply to drug formulations based on polymeric nanoparticles. In this study, we obtained a series of particles based on biodegradable poly(D,L-lactide)-b-poly(ethylene glycol) (P(D,L)LA
n -b-PEG113 ) copolymers with varied hydrophobic P(D,L)LA block length n from 50 to 1230 monomer units stabilized by poly(vinyl alcohol) (PVA) by a simple "oil-in-water" emulsion method. We found that nanoparticles of P(D,L)LAn -b-PEG113 copolymers with relatively short P(D,L)LA block (n ≤ 180) are prone to aggregate in water. P(D,L)LAn -b-PEG113 copolymers with n ≥ 680 can form spherical unimodal particles with values of hydrodynamic diameter less than 250 nm and polydispersity less than 0.2. The aggregation behavior of P(D,L)LAn -b-PEG113 particles was elucidated in terms of tethering density and conformation of PEG chains at the P(D,L)LA core. Docetaxel (DTX) loaded nanoparticles based on P(D,L)LA680 -b-PEG113 and P(D,L)LA1230 -b-PEG113 copolymers were formulated and studied. It was observed that DTX-loaded P(D,L)LAn -b-PEG113 (n = 680, 1230) particles are characterized by high thermodynamic and kinetic stability in aqueous medium. The cumulative release of DTX from the P(D,L)LAn -b-PEG113 (n = 680, 1230) particles is sustained. An increase in P(D,L)LA block length results in a decrease in DTX release rate. The in vitro antiproliferative activity and selectivity studies revealed that DTX-loaded P(D,L)LA1230 -b-PEG113 nanoparticles demonstrate better anticancer performance than free DTX. Favorable freeze-drying conditions for DTX nanoformulation based on P(D,L)LA1230 -b-PEG113 particles were also established. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF