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Recipes for traditional and sour non-alcoholic beers were developed in this study employing a special yeast species Saccharomycodes ludwigii. They were characterized for their basic physicochemical properties, antioxidative activity as well as subjected to the quantitative and qualitative analysis of their biologically-active compounds, and to the sensory assessment. Sour non-alcoholic beers were brewed with the addition of juice from fruits of red-colored Cornelian cherry (Cornus mas L.) variety, which are characterized by naturally sour taste and aroma. Ethyl alcohol content in the beers manufactured ranged from 0.41%v/v in traditional non-alcoholic beers to 0.43%v/v in sour non-alcoholic beers. The final products had a low energy value, ranging from 116 to 148 kcal/500 mL of beer. The sour beers had several times higher antioxidative potential and significantly higher polyphenols concentration compared to the control ones. In addition, they were rich in anthocyanins and iridoids, and presented novel sensory attributes., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The aim of this study was to determine the possibility of the use of Cornelian cherry (CC) juices in brewing technology. We analyzed basic physicochemical properties, concentration of polyphenols and iridoids, and antioxidative activity of brewed beer. The concentration of total polyphenols (F-C) in CC beer ranged from 398.1 to 688.7 mg GAE/L beer. The antioxidative activity measured with the DPPH and FRAP assays was the highest in the beer with the addition of juice from red-fruit CC cultivar. Among the identified iridoids, loganic acid was the predominating compounds and its highest concentration, accounting for 184.6 mg LA/L beer, was found in the beer with juice made of coral-fruit CC cultivar. The identified polyphenols included anthocyanins and flavonol derivatives. The novelty of this study was to brewed beers containing compounds from the group of iridoids., (Copyright © 2019. Published by Elsevier Ltd.)

In this study, we investigated the effect of Cornelian cherry cultivars differing in fruit color ("Yantaryi"-yellow fruits, "Koralovyi"-coral fruits, "Podolski"-red fruits) on physicochemical characteristics, antioxidative properties, and contents of iridoids and polyphenols in meads with the addition of juices made of their fruits. " Trójniak " type meads (1:2 honey to water volume) were manufactured from multifloral honey, to which Cornelian cherry fruit juice was added before fermentation. Concentrations of individual iridoids and polyphenols were determined using HPLC analysis with a thermostat refractometric detector, model RID-10A. The total polyphenol content was determined based on testing with Folin⁻Ciocalteu (F-C) reagent, whereas the antioxidative properties were determined using DPPH • (2,2-Diphenyl-2-picryl-hydrazyl), ABTS •⁺ (2,2'-Azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid), and FRAP (ferric reducing antioxidant power) assays. Cultivar of Cornelian cherry fruits influenced both the antioxidative properties and the concentrations of polyphenols and iridoids. The highest concentration of total polyphenols (F-C), accounting for 898.7 mg gallic acid (GAE)/L, was determined in the mead with juice made of red fruits; this mead was also characterized by the strongest antioxidative capabilities measured with ABTS •⁺ and FRAP assays. Among the iridoids determined in the Cornelian cherry meads, loganic acid was found to prevail and its highest concentration, reaching 77.8 mg loganic acid (LA)/L mead, was determined in the mead with the coral-fruit juice. Study results indicate that Cornelian cherry meads have a high content of biologically active iridoids and phenolic acids which display valuable antioxidative properties.

We analyzed the effect of Cornelian cherry varieties differing in fruit color ('Yantaryi'-yellow fruits, 'Koralovyi'-coral fruits, 'Podolski'-red fruits) and the production method on the physicochemical and antioxidative properties of Cornelian cherry vinegars, and on their content of iridoids and polyphenols. Acetic fermentation was conducted by two methods: I) single-stage (spontaneous) acetic fermentation, without inoculation with microorganisms, and II) two-stage fermentation in which the first stage involved the use of Saccharomyces bayanus -Safspirit fruit yeast for alcoholic fermentation, and the second one included spontaneous acetic fermentation. Acetic acid, glycerol, individual iridoids, phenolic acids, flavonols, and anthocyanins were quantified by a high-performance liquid chromatography (HPLC) method. The antioxidative activity was determined based on the following tests: 2,2-Diphenyl-2-picryl-hydrazyl (DPPH • ), 2,2'-Azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS •+ ), and ferric reducing antioxidant power (FRAP), while the total polyphenols content was determined using the Folin-Ciocialteu (F-C) reagent test. Both the Cornelian cherry variety and vinegar production method affected the antioxidative properties as well as concentrations of iridoids and polyphenols in the finished product. The concentration of total polyphenols (F-C) in vinegars ranged from 326.60 to 757.27 mg gallic acids equivalents (GAE)/100 mL vinegar, whereas the antioxidative activity assayed with the DPPH • and FRAP methods was the highest in the vinegars produced from the coral and red varieties of Cornelian cherry with the two-stage method. Loganic acid predominated among the identified iridoids, reaching a concentration of 185.07 mg loganic acid (LA)/100 mL in the vinegar produced in the two-stage fermentation from the coral-fruit variety. Caffeoylquinic acid derivatives were the main representatives among the identified phenolic compounds. The results of this study demonstrate Cornelian cherry vinegars to be rich sources of biologically-active iridoids and phenolic compounds with antioxidative properties., Competing Interests: The authors declare no conflict of interest.