1. Selective downregulation of retinoic acid-inducible gene I within the intestinal epithelial compartment in Crohn's disease.
- Author
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Funke B, Lasitschka F, Roth W, Penzel R, Meuer S, Saile M, Gretz N, Sido B, Schirmacher P, and Autschbach F
- Subjects
- Adult, Aged, Biomarkers metabolism, Blotting, Western, Case-Control Studies, Cohort Studies, Colitis, Ulcerative metabolism, Crohn Disease metabolism, DEAD Box Protein 58, DEAD-box RNA Helicases metabolism, Female, Gene Expression Profiling, Humans, Immunoenzyme Techniques, Male, Middle Aged, Oligonucleotide Array Sequence Analysis, Prognosis, RNA, Messenger genetics, Receptors, Immunologic, Reverse Transcriptase Polymerase Chain Reaction, Biomarkers analysis, Colitis, Ulcerative genetics, Crohn Disease genetics, DEAD-box RNA Helicases genetics, Intestinal Mucosa metabolism
- Abstract
Background: A defective innate immune response may contribute to the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC). Employing a global gene expression analysis, this study was aimed at identifying specifically regulated genes within the epithelial compartment in inflammatory bowel disease (IBD)., Methods: The epithelial fraction of human ileal mucosa samples from surgical specimens was obtained by laser microdissection. Gene expression was examined by global expression profiling (n = 18, Affymetrix), quantitative reverse-transcription polymerase chain reaction (RT-PCR) (n = 35), immunoblot analysis (n = 9), and immunohistochemistry (n = 25)., Results: Global expression profiling revealed a pronounced downregulation of the retinoic acid-inducible gene I (RIG-I) within the epithelial layer of the ileum in patients with CD but not with UC. The downregulation of RIG-I was confirmed by quantitative RT-PCR, immunoblot analysis, and immunohistochemistry., Conclusions: Epithelial downregulation of RIG-I, a known pattern recognition receptor for viral components, might contribute to alterations of the innate mucosal immune response, particularly in CD., (Copyright © 2011 Crohn's & Colitis Foundation of America, Inc.)
- Published
- 2011
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