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(1) The Environmental Trace Gases Monitoring Instrument-2(EMI-2) is a high-quality spaceborne imaging spectrometer that launched in September 2021. To evaluate its radiometric calibration performance in-flight, the UV2 and VIS1 bands of EMI-2 were cross-calibrated by the corresponding bands (band3 and band4) of TROPOMI over the pseudo-invariant calibration site Dome C. (2) After angle limitation and cloud filtering of the Earth radiance data measured by EMI-2 and TROPOMI over Dome C, the top of atmosphere (TOA) reflectance time series were calculated. The spectral adjustment factors (SAF) were derived from the solar spectrum measured by the sensor to minimize the uncertainties caused by the different spectral response functions (SRF) of sensors. In addition, a correction method based on the radiative transfer model (RTM) SCIATRAN was used to suppress unaccounted angular dependence of atmospheric scattering. The radiation performance of EMI-2 is evaluated using the TOA reflectance ratio of EMI-2 and TROPOMI, combining the SAF correction and RTM-based correction methods. (3) It was shown that the time series trending of the TOA reflectance ratio between EMI-2 measurements and TROPOMI demonstrate flat characteristics and strong correlation. The mean reflectance ratios range from 0.998 to 1.09. The standard deviation of the reflection ratio is less than 3%. For 328 nm, 335 nm, 340 nm, 460 nm, and 490 nm, the mean values are close to one, and the relative radiometric bias estimated through EMI-2 and TROPOMI intercalibration is less than 3%, and for other wavelengths, the biases are less than 6%, except for 416 nm, which behaves higher than 7%. The cross-calibration results show that the radiometric calibration of EMI-2 is within the relative accuracy requirement. [ABSTRACT FROM AUTHOR]