14 results on '"Matencio, Adrián"'
Search Results
2. Hyper-Branched Cyclodextrin-Based Polymers as Anticoagulant Agents: In Vitro and In Vivo Studies.
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Monfared, Yousef Khazaei, Mahmoudian, Mohammad, Hoti, Gjylije, Bisericaru, Daniel Mihai, Caldera, Fabrizio, Cavalli, Roberta, Zakeri-Milani, Parvin, Matencio, Adrián, and Trotta, Francesco
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ANTICOAGULANTS ,BLOOD coagulation ,COORDINATION polymers ,POLYMERS ,DRUG carriers - Abstract
This study tested the anticoagulant effect of cyclodextrin (CD) hyper-branched-based polymers (HBCD-Pols). These polymers were synthesized and tested for their coagulant characteristics in vitro and in vivo. Due to their polymeric structure and anionic nature, the polymers can chelate Ca
2+ , reducing the free quantity in blood. HBCD-Pol increased the blood clotting time, PT, and aPTT 3.5 times over the control, showing a better effect than even ethylenediaminetetraacetic acid (EDTA), as occured with recalcification time as well. A titration of HBCD-Pol and EDTA showed exciting differences in the ability to complex Ca2+ between both materials. Before executing in vivo studies, a hemocompatibility study was carried out with less than 5% red blood cell hemolysis. The fibrinogen consumption and bleeding time were analyzed in vivo. The fibrinogen was considerably decreased in the presence of HBCD-Pol in a higher grade than EDTA, while the bleeding time was longer with HBCD-Pols. The results demonstrate that the anticoagulant effect of this HBCD-Pol opens novel therapy possibilities due to the possible transport of drugs in this carrier. This would give combinatorial effects and a potential novel anticoagulant therapy with HBCD-Pol per se. [ABSTRACT FROM AUTHOR]- Published
- 2022
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3. Molecular encapsulation and bioactivity of gnetol, a resveratrol analogue, for use in foods.
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Navarro‐Orcajada, Silvia, Conesa, Irene, Matencio, Adrián, García‐Carmona, Francisco, and López‐Nicolás, José Manuel
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MICROENCAPSULATION ,RESVERATROL ,CHEMICAL industry ,RADICAL cations ,STILBENE ,FUNCTIONAL foods - Abstract
BACKGROUND Gnetol is a stilbene whose characterization and bioactivity have been poorly studied. It shares some bioactivities with its analogue resveratrol, such as anti‐inflammatory, anti‐thrombotic, cardioprotective and anti‐cancer activities. However, the low solubility of stilbenes may limit their potential applications in functional foods. Encapsulation in cyclodextrins could be a solution. RESULTS: The antioxidant activity of gnetol was evaluated by 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) radical cation and ferric reducing antioxidant power methods (Trolox equivalents 13.48 μmol L−1 and 37.08 μmol L−1 respectively at the highest concentration) and it was higher than that of resveratrol, and depending on the method, similar or higher to that of oxyresveratrol. Spectrophotometric and spectrofluorimetric characterization of gnetol is published for the first time. Moreover, its water solubility was determined and improved almost threefold after its molecular encapsulation in cyclodextrins, as well as its stability after storage for a week. A physicochemical and computational study revealed that cyclodextrins complex gnetol in a 1:1 stoichiometry, with better affinity for like 2‐hydroxypropyl‐β‐cyclodextrin (KF = 4542.90 ± 227.15 mol−1 L). Temperature and pH affected the encapsulation constants. CONCLUSION: These results could increase interest of gnetol as an alternative to the most studied stilbene, resveratrol, as well as aid in the development of more stable inclusion complexes that improve its aqueous solubility and stability so that it can be incorporated into functional foods. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Dextrin-Based Nanohydrogels for Rokitamycin Prolonged Topical Delivery.
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Tannous, Maria, Lucia Appleton, Silvia, Hoti, Gjylije, Caldera, Fabrizio, Argenziano, Monica, Monfared, Yousef Khazaei, Matencio, Adrián, Trotta, Francesco, and Cavalli, Roberta
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DEXTRINS ,HYDROGELS ,CYCLODEXTRINS ,POLYMERS ,ANTIBIOTICS - Abstract
Macrolides are widely used antibiotics with a broad spectrum of activity. The development of drug carriers to deliver this type of antibiotics has attracted much research. The present study aims at developing new swellable dextrin-based nanohydrogels for the topical delivery of rokitamycin, as model macrolide. Rokitamycin is a synthetic analogous of macrolides with advantageous characteristics as far as bacterial uptake and post-antibiotic effect are concerned. It is also indicated for the treatment of severe infections caused by Acanthamoeba and for topical infections. The nanohydrogels have been prepared from two types of cross-linked polymers obtained by using β-cyclodextrin or Linecaps
® was provided by the Roquette Italia SPA (Cassano Spinola, Al, Italy) as building blocks. The cross-linked polymers have been then formulated into aqueous nanosuspensions refined and tuned to achieve the incorporation of the drug. Cross-linked β-cyclodextrin (β-CD) and Linecaps® (LC) polymers formed dextrin-based nanohydrogels with high swelling degree and mucoadhesion capability. Rokitamycin was loaded into the nanohydrogels displaying an average size around 200 nm with negative surface charge. In vitro kinetic profiles of free and loaded drug in nanohydrogels were compared at two pH levels. Interestingly, a sustained and controlled release was obtained at skin pH level due to the high degree of swelling and a pH responsiveness possibly. The results collected suggest that these nanohydrogels are promising for the delivery of rokitamycin and may pave the way for the topical delivery of other macrolide antibiotics. [ABSTRACT FROM AUTHOR]- Published
- 2022
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5. Magnetic Composites of Dextrin-Based Carbonate Nanosponges and Iron Oxide Nanoparticles with Potential Application in Targeted Drug Delivery.
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Caldera, Fabrizio, Nisticò, Roberto, Magnacca, Giuliana, Matencio, Adrián, Khazaei Monfared, Yousef, and Trotta, Francesco
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Magnetically driven nanosponges with potential application as targeted drug delivery systems were prepared via the addition of magnetite nanoparticles to the synthesis of cyclodextrin and maltodextrin polymers crosslinked with 1,1′-carbonyldiimidazole. The magnetic nanoparticles were obtained separately via a coprecipitation mechanism involving inorganic iron salts in an alkaline environment. Four composite nanosponges were prepared by varying the content of magnetic nanoparticles (5 wt% and 10 wt%) in the cyclodextrin- and maltodextrin-based polymer matrix. The magnetic nanosponges were then characterised by FTIR, TGA, XRD, FESEM, and HRTEM analysis. The magnetic properties of the nanosponges were investigated via magnetisation curves collected at RT. Finally, the magnetic nanosponges were loaded with doxorubicin and tested as a drug delivery system. The nanosponges exhibited a loading capacity of approximately 3 wt%. Doxorubicin was released by the loaded nanosponges with sustained kinetics over a prolonged period of time. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Cyclodextrin-Based Nanosponges as Perse Antimicrobial Agents Increase the Activity of Natural Antimicrobial Peptide Nisin.
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Khazaei Monfared, Yousef, Mahmoudian, Mohammad, Hoti, Gjylije, Caldera, Fabrizio, López Nicolás, José Manuel, Zakeri-Milani, Parvin, Matencio, Adrián, and Trotta, Francesco
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ANTIMICROBIAL peptides ,ANTI-infective agents ,NISIN ,CYCLODEXTRINS ,DRUG resistance in bacteria ,BACTERIAL diseases - Abstract
At present, antibiotic resistance is considered a real problem. Therefore, for decades scientists have been looking for novel strategies to treat bacterial infections. Nisin Z, an antimicrobial peptide (AMP), can be considered an option, but its usage is mainly limited by the poor stability and short duration of its antimicrobial activity. In this context, cyclodextrin (CD)-based nanosponges (NSs), synthesized using carbonyldiimidazole (CDI) and pyromellitic dianhydride (PMDA), were chosen for nisin Z loading. To determine the minimum inhibitory of nisin Z loaded on CD-NS formulations, agar well diffusion plates were used. Then, the bactericide concentrations of nisin Z loaded on CD-NS formulations were determined against Gram-positive (Staphylococcus aureus) and -negative (Escherichia coli) bacteria, using microdilution brain heart infusion (BHI) and tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The minimum and bactericide inhibitory values of the nisin complex with NSs were potentially decreased against both bacteria, compared with the nisin-free sample, while the nisin complex with β-CD showed lower antibacterial activity. The antimicrobial effect was also demonstrated by free NSs. Furthermore, the total viable counts (TVCs) antibacterial experiment indicated that the combination of nisin Z in both PMDA and CDI β-CD-based NSs, especially CDI, can provide a better conservative effect on cooked chicken meat. Generally, the present study outcomes suggest that the cross-linked β-CD-based NSs can present their own antimicrobial potency or serve as promising carriers to deliver and enhance the antibacterial action of nisin Z. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Application of a simple methodology to analyze Hydroxypropyl-β-Cyclodextrin in urine using HPLC–LS in early Niemann–Pick disease type C patient.
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Matencio, Adrián, Alcaráz-Gómez, María Agustina, García-Carmona, Francisco, Arias, Begoña, and López-Nicolás, José Manuel
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CYCLODEXTRINS , *URINALYSIS , *NIEMANN-Pick diseases , *DISEASE progression , *CHILDREN'S health , *HIGH performance liquid chromatography - Abstract
A new methodology, based on high resolution liquid chromatography with light scatterin detector, is applied to analyze Hydroxypropyl-beta-Cyclodextrin (HPβCD) in urine samples of a child affected by Niemann-Pick Type C disease. The treatment not only stopped disease progression, but has also increased the life expectancy and quality of our patient. The pharmacokinetic of HPβCD in the patient was studied with a 92.8% of HPβCD recovered. At 88 h, no HPβCD was found in the urine. During the treatment, HPβCD has not shown toxicity. Before application of the new treatment, injections were given every two weeks but, we have demonstrated that this can be increased to every four days. [ABSTRACT FROM AUTHOR]
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- 2018
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8. Separating and Identifying the Four Stereoisomers of Methyl Jasmonate by RP-HPLC and using Cyclodextrins in a Novel Way.
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Matencio, Adrián, Bermejo‐Gimeno, Mario J., García‐Carmona, Francisco, and López‐Nicolás, José Manuel
- Abstract
Introduction Several authors have reported on the different bioactivities of methyl jasmonate (MeJA) stereoisomers. However, no simple, precise and cheap method for separating and identifying them using reversed-phase high performance liquid chromatography (RP-HPLC) has been developed. Objective (1) To create a simple, precise and cheap method for separating and identifying the four stereoisomers present in commercial racemic mixtures of MeJA and (2) to identify the four stereoisomers using molecular docking techniques and coinjection. Materials and Methods - RP-HPLC using a 250 mm C18 column and different proportions of cyclodextrins (CDs) and organic solvents was applied to a commercial sample of racemic MeJA. Results The results show that the best conditions for separating the MeJA stereoisomers are: 20% methanol in the mobile phase, a temperature of 45 °C and a 16 mM concentration of methyl- β-cyclodextrin (M-β-CD). A simple C18 250 mm column and a flow rate of 1.25 mL/min were used. The reduction in the retention time of MeJA observed when M-β-CD is added to the mobile phases was used to determine the complexation constants of the guest/CD complex and compared with the obtained when other CDs were used. The K
F for M-β-CD (117.49 ± 5.9 1/M) was obtained with a 1:1 stoichiometry. The four stereoisomers were identified by molecular docking techniques and coinjection of a commercially available rosemary essential oil. Conclusion The new method identified and classified the four stereoisomers of MeJA in the following ordination: (−)epiMeJA, (−)MeJA; (+)MeJA and (+)epiMeJA. These results could be used to improve the elicitation of cell cultures with only the best isomer. Copyright © 2016 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]- Published
- 2017
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9. Aggregation of t10,c12 conjugated linoleic Acid in presence of natural and modified cyclodextrins. A physicochemical, thermal and computational analysis.
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Matencio, Adrián, García-Carmona, Francisco, and López-Nicolás, José Manuel
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CONJUGATED linoleic acid , *CLUSTERING of particles , *CYCLODEXTRINS , *THERMAL analysis , *STOICHIOMETRY - Abstract
In this work the aggregation behavior of t10,c12 Conjugated linoleic acid (t10,c12-CLA) is presented for first time. The results show a c.m.c. of 25 μM at pH 8 and 25 °C. The encapsulation process with cyclodextrins (CDs) presented a 1:1 stoichiometry in all cases studied but the complexation constants were strongly dependent on the type of CDs used, the pH and temperature. Hydroxypropyl-beta-Cyclodextrin (HPβCD) was the best CD studied for encapsulating t10,c12-CLA. The resulting t10,c12-CLA-HPβCD complex showed a very high dependency on pH, which explains why a pK a of 4.08 was found for first time, which was very close to the simulated value. Furthermore, the effect of temperature on the t10,c12-CLA-HPβCD was studied. The complexation constant (K F ) showed an increase behavior with the temperature. In addition, molecular docking calculations provided further insights into how the different interactions influence the complexation constant. Finally, a comparative study with rumenic acid, an isomer, was carried out. [ABSTRACT FROM AUTHOR]
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- 2017
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10. Physicochemical, thermal and computational study of the encapsulation of rumenic acid by natural and modified cyclodextrins.
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Matencio, Adrián, Hernández-Gil, Carlos Javier García, García-Carmona, Francisco, and López-Nicolás, José Manuel
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LINOLEIC acid , *CYCLODEXTRINS , *THERMAL properties , *MICROENCAPSULATION , *COMPUTATIONAL chemistry , *PHYSICAL & theoretical chemistry - Abstract
In this work the aggregation behavior of Rumenic acid (RA) is presented for the first time. The results point to a c.m.c. of 35 μM at pH 8 and 25 °C. This behavior can be modified by introducing CDs into the system to encapsulate the RA. The encapsulation process presented a 1:1 stoichiometry in all the cases studied but the complexation constants were strongly dependent on the type of CDs used, the pH and temperature. Firstly, the effect of the type of CD on the encapsulation process was studied. Among the natural and modified CDs analyzed HPβCD was the best for encapsulating RA. The pK a determined for RA was 4.31. The K F showed different behavior below and above 25 °C due to changes in the stoichiometry. Finally, molecular docking calculations provided further insights into how the different interactions influence the complexation constant. [ABSTRACT FROM AUTHOR]
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- 2017
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11. A physicochemical, thermodynamical, structural and computational evaluation of kynurenic acid/cyclodextrin complexes.
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Matencio, Adrián, Caldera, Fabrizio, Rubin Pedrazzo, Alberto, Khazaei Monfared, Yousef, K. Dhakar, Nilesh, and Trotta, Francesco
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INCLUSION compounds , *MOLECULAR docking , *CYCLODEXTRINS , *ACIDS , *ENTHALPY , *THERMODYNAMIC functions - Abstract
• The interaction of KYNA and several cyclodextrins (CDs) was studied. • The modified HPβ-CD showed the strongest complexation constant (KF) • The results showed high dependence on pH and temperature. In this work, the interaction between Kynurenic acid (KYNA) and several natural and modified cyclodextrins (CDs) is carried out. Among all the CD tested, HPβ-CD showed the strongest complexation constant (K F), with a value of 270.94 ± 29.80 M−1. Between natural (α- and β-) CDs, the complex of KYNA with β-CD was the most efficient. The inclusion complex of KYNA with CDs showed a strong influence of pH and temperature. The K F value decreased at high pH values, when the pK a was passed. Moreover, an increase of the temperature caused a decrease in the K F values. The thermodynamic parameters of the complexation (Δ H °, Δ S ° and Δ G °) were studied with negative entropy, enthalpy and spontaneity of the process at 25 °C. Moreover, the inclusion complex was also characterized using FTIR and TGA. Finally, molecular docking calculations provided different interactions and their influence in the complexation constant. [ABSTRACT FROM AUTHOR]
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- 2021
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12. Nanoparticles of betalamic acid derivatives with cyclodextrins. Physicochemistry, production characterization and stability.
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Matencio, Adrián, Guerrero-Rubio, M. Alejandra, Gandía-Herrero, Fernando, García-Carmona, Francisco, and López-Nicolás, José Manuel
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CYCLODEXTRIN derivatives , *ACID derivatives , *MOLECULAR docking , *PH effect , *BIOACTIVE compounds - Abstract
The use of betalains as bioactive compounds has been widely investigated. However, their use as a functional food requires a more exhaustive study because of their photosensitivity and poor stability. For this reason, this works studies the stabilization of two highly bioactive betalain derivatives, phenylethylamine-betaxanthin (Ph-Bx) and indoline-betacyanin (In-Bc) by nanoencapsulation in natural a modified cyclodextrins (CDs). Methyl-β-CD (Mβ-CD) showed the best results for Ph-Bx while Hydroxypropyl-β-CD (HPβ-CD) was better in the case of In-Bc. In addition, the effect of pH and temperature on encapsulation was studied finding that the encapsulation constant (K F) value increased as the pH decreased in the case of Ph-Bx but not in the case of In-Bc. The K F decreased as the temperatures increased. The thermodynamic parameters, ΔH°, ΔS° and ΔG°, were also calculated. Molecular docking provided further insight into how the different interactions influenced the encapsulation constant. The results show that the presence of CDs during the biotechnological synthesis of Ph-Bx and In-Bc increased production by 51 and 26%, respectively, and also increased the stability of both derivatives. This study represents a first step towards increasing the production of this type of compound for introduction into functional foods. Image 1 • Two betalains derivatives were nanoencapsulated using natural and modified cyclodextrin. • Methyl-β-CD (Mβ-CD) showed the best results for phenylethylamine-betaxanthin. • Hydroxypropyl-β-CD (HPβ-CD) was better in the case of indoline-betacyanin. • The complexation showed an increase of stability and bioproduction for both derivatives. [ABSTRACT FROM AUTHOR]
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- 2021
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13. The use of cyclodextrins as solubility enhancers in the ORAC method may cause interference in the measurement of antioxidant activity.
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Navarro-Orcajada, Silvia, Conesa, Irene, Matencio, Adrián, Rodríguez-Bonilla, Pilar, García-Carmona, Francisco, and López-Nicolás, José Manuel
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CYCLODEXTRINS , *SOLUBILITY , *REACTIVE oxygen species , *RESVERATROL , *INCLUSION compounds , *ANTIOXIDANTS - Abstract
The ability of cyclodextrins to enhance the water solubility of lipophilic compounds is used to modify the water-based Oxygen Radical Absorbance Capacity (ORAC) method to measure antioxidant activity in vitro. However, the use of these solubility enhancers may alter fluorescent readings, which has led to contradictory results being described in the literature. The low specificity of these oligosaccharides and their controlled release effect can result in cyclodextrins forming inclusion complexes with other reagents in the assay, changing the kinetics. In this study, the cause of cyclodextrins' interference in the ORAC method is evaluated through a physicochemical and computational approach. Cyclodextrins showed a clear increase in the fluorescent signal both in the presence and absence of the antioxidant oxyresveratrol, the precise effect being dependent on the type and concentration of cyclodextrin. Although the glucidic nature of cyclodextrins could play a minimal role in this effect, it seems that the main cause was the encapsulation of other substrates in the reaction, fluorescein and AAPH. [Display omitted] • Different types of cyclodextrins can modify ORAC readings in a dose-dependent manner. • This effect is mainly due to the ability of cyclodextrins to encapsulate other molecules. • Fluorescein and AAPH can be trapped in the cyclodextrin cavity, as well as the antioxidant. • The use of cyclodextrins as solubility enhancers in the ORAC method is discouraged. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Oral supplementation of solvent-free kynurenic acid/cyclodextrin nanosponges complexes increased its bioavailability.
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Monfared, Yousef Khazaei, Pedrazzo, Alberto Rubin, Mahmoudian, Mohammad, Caldera, Fabrizio, Zakeri-Milani, Parvin, Valizadeh, Hadi, Cavalli, Roberta, Matencio, Adrián, and Trotta, Francesco
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CYCLODEXTRINS , *DIETARY supplements , *BIOAVAILABILITY , *OXIDANT status , *ORAL drug administration , *TIME pressure , *FORMYLATION - Abstract
Many nutraceuticals present problems due to their poor water solubility or stability, which prevents the final bioactivity achievement. For that reason, the oral administration of KYNA complexed with HPβ-CD and βNS-CDI nanosponges was evaluated in mice. The solvent-free technology was used to prepare the complexes in a complete comparison between kneading in ball milling and classical inclusion complex preparation. The solvent-free ones showed higher strength and efficiency with ball milling, considerably reducing time. A 50 mg KYNA/kg/day dosage was orally administered in formulations showing a higher bioavailability when the nutraceutical was complexed with βNS-CDI compared to HPβ-CD and free KYNA, respectively. Several antioxidant statuses demonstrated a higher global antioxidant level perfectly related to bioavailability. Finally, the formulation of KYNA reduced the temporal oxidative stress damage in the kidney and liver, making βNS-CDI the best formulation. These results suggest an important future application of cyclodextrin-based nanosponges for the oral delivery of nutraceuticals and their stabilization. [Display omitted] • A comparison between conventional and ball milling complexes was carried out. • Higher encapsulation strength, velocity, and efficiency were found with ball milling. • βNS-CDI showed higher bioavailability than HPβ-CD complexes. • The higher KYNA dosage increased the total antioxidant status of mice. • The complexes form reduced the KYNA effect in the kidney and liver. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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