1. RS cyclophilins: identification of an NK-TR1-related cyclophilin.
- Author
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Nestel FP, Colwill K, Harper S, Pawson T, and Anderson SK
- Subjects
- Amino Acid Isomerases chemistry, Amino Acid Isomerases genetics, Amino Acid Sequence, Animals, Arginine chemistry, Base Sequence, Carrier Proteins chemistry, Carrier Proteins genetics, Cloning, Molecular, DNA, Complementary, Gene Expression, Humans, Killer Cells, Natural chemistry, Mice, Molecular Sequence Data, Protein Serine-Threonine Kinases metabolism, Protein-Tyrosine Kinases metabolism, RNA Splicing, Receptors, Natural Killer Cell, Serine chemistry, T-Lymphocytes chemistry, Amino Acid Isomerases isolation & purification, Blood Proteins chemistry, Carrier Proteins isolation & purification, Cyclophilins
- Abstract
We report the isolation of a large cyclophilin protein containing RS (arginine-serine) repeats from a yeast two-hybrid screen using ClK (CDC28/cdc2-like kinase) as a probe. This Clk associating RS-cyclophilin (CARS-Cyp) possesses 39% homology to the NK-TR1 (natural killer tumor recognition protein-1) we have previously characterized (Anderson et al. (1993) Proc. Natl. Acad. Sci. USA 90 (1993) 542-546). CARS-Cyp is expressed in a variety of tissues and cell types, and codes for a protein with a predicted mass of 89 kDa containing a cyclophilin-related domain, two Nopp140 (nucleolar phosphoprotein of 140 kDa)-related domains, and a large RS domain. The RS-cyclophilins, a novel class of proteins, may play an important role in the regulation of pre-mRNA splicing.
- Published
- 1996
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