1. A mitochondria-targeted single fluorescence probe for separately and continuously visualizing H 2 S and Cys with multi-response signals.
- Author
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Zhao X, Ji H, Hasrat K, Misal S, He F, Dai Y, Ma F, and Qi Z
- Subjects
- Animals, Colorimetry, Coumarins chemical synthesis, Coumarins chemistry, Cysteine chemistry, Fluorescent Dyes chemical synthesis, Hep G2 Cells, Humans, Hydrogen Sulfide chemistry, Indoles chemical synthesis, Indoles chemistry, Limit of Detection, Microscopy, Confocal, Microscopy, Fluorescence, Zebrafish, Cysteine analysis, Fluorescent Dyes chemistry, Hydrogen Sulfide analysis, Mitochondria metabolism
- Abstract
Hydrogen sulfide and cysteine are momentous endogenous regulators of many physiological processes and maintain a dynamic balance of redox in living organisms. To investigate the inter-relationship of them in vivo, there is a pressing need to develop analytical molecular tools to identify related biomolecules. We construct a mitochondria-targeted single fluorescence probe (Mit-CM) for separately and continuously visualizing H
2 S, Cys and H2 S/Cys with multi-response fluorescence signals. Mit-CM has the following advantages: (Ⅰ) colorimetric and ratiometric: two well-separated emission bands can ensure accurate detection of the analyte and significant color changes contribute to rapid identification of the analyte by the naked eye; (Ⅱ) mitochondrial localization: study the physiological functions of H2 S and Cys in mitochondria; (Ⅲ) separate and continuous detection of H2 S and Cys: reveal the inter-relationship and interconversion of them in biological system. Moreover, the desirable attributes of low cytotoxicity, better biocompatibility and excellent mitochondria enrichment ability indicate that Mit-CM can be employed to achieve detection and observe distribution of H2 S, Cys and H2 S/Cys in living organism., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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