1. Simple and reliable preparation of immunodiagnostic antigens for Taenia solium cysticercosis.
- Author
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Sako Y, Itoh S, Okamoto M, Nakaya K, and Ito A
- Subjects
- Animals, China, Chromatography, Ion Exchange, Cysticercosis immunology, Cysticercosis parasitology, Developing Countries, Ecuador, Humans, Larva chemistry, Larva immunology, Molecular Weight, Reagent Kits, Diagnostic, Swine, Swine Diseases immunology, Swine Diseases parasitology, Taenia solium chemistry, Taenia solium immunology, Antigens, Helminth analysis, Cysticercosis diagnosis, Cysticercosis veterinary, Immunoassay, Swine Diseases diagnosis, Taenia solium isolation & purification
- Abstract
SUMMARY Cysticercosis caused by infection with the larval stage of Taenia solium is an important cause of neurological disease worldwide and immunodiagnosis is important for the control and elimination of cysticercosis. In the present study, we established a simple and reliable preparation of immunodiagnostic low-molecular-weight antigens (LMWAgs) from T. solium cyst fluids by a cation-exchange chromatography (CEC). Banding patterns of LMWAgs on SDS-PAGE were different between isolates from Ecuador and China. All cysticercosis patient sera and some echinococcosis patient sera recognized both LMWAgs by enzyme-linked immunosorbent assay (ELISA), but sera from healthy persons were not positive. There was no statistical difference in immunodiagnostic performance of LMWAgs prepared from different geographical isolates. These results indicated that these novel immunodiagnostic antigen preparations could contribute the control and prevention of cysticercosis in endemic areas, especially developing countries.
- Published
- 2013
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