1. Redox‐State‐Mediated Regulation of Cytochrome c Release in Apoptosis Revealed by Surface‐Enhanced Raman Scattering on Nickel Substrates.
- Author
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Zhu, Jinyu, Jiang, Muwei, Ma, Hao, Zhang, Haijing, Cheng, Weina, Li, Junbo, Cai, Linjun, Han, Xiao Xia, and Zhao, Bing
- Subjects
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RAMAN scattering , *CYTOCHROME c , *NICKEL , *APOPTOSIS , *MOLECULAR dynamics , *RAMAN spectroscopy - Abstract
The interaction of cytochrome c (Cyt c) with cardiolipin (CL) is believed to play an important role in the initial events of apoptosis. Herein, we investigate the structural changes of CL‐bound Fe2+Cyt c and the correlation with Cyt c release through surface‐enhanced Raman spectroscopy (SERS) on nickel substrates. The SERS results together with molecular dynamics simulation reveal that Fe2+Cyt c undergoes autoxidation and a relatively larger conformational alteration after binding with CL, inducing higher peroxidase activity of Cyt c and higher permeability of the CL membrane compared with those induced by the Fe3+Cyt c. The proapoptotic activity and SERS effect of the Ni nanostructures allow the in situ study of the redox‐state‐dependent Cyt c release from isolated mitochondria, which reveals for the first time that the ferrous state of Cyt c most likely plays a more important role in triggering apoptosis. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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