1. Transcriptional regulation of the yeast cytochrome c gene.
- Author
-
Zitomer RS, Montgomery DL, Nichols DL, and Hall BD
- Subjects
- DNA, Recombinant, Genes, Glucose metabolism, Nucleic Acid Hybridization, RNA, Messenger metabolism, Raffinose metabolism, Cytochrome c Group genetics, Saccharomyces cerevisiae genetics, Transcription, Genetic
- Abstract
DNA from the cloned yeast iso-1-cytochrome c, cycl, gene was used in a hybridization assay to measure levels and rates of synthesis of cycl RNA. Derepressed cells synthesized cycl RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cycl gene was repressed within 2.5 min. The half-life of hybridizable cycl RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cycl gene is subject to transcriptional regulation.
- Published
- 1979
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