1. Identification of monoclonal antibodies that cross-react with cytokines from different animal species
- Author
-
Pedersen, L.G., Castelruiz, Y., Jacobsen, S., and Aasted, B.
- Subjects
- *
MONOCLONAL antibodies , *CYTOKINES , *FLOW cytometry - Abstract
Eleven monoclonal antibodies specific for ovine, bovine and human cytokines were investigated by flow cytometry for cross-reactivities with cytokines produced by peripheral blood mononuclear cells (PBMCs) from sheep, cattle, goat, swine, horse, dog, mink, rabbit and human. Four antibodies specific for IL-4, IL-8, IFN-γ and TNF-α cross-reacted with cytokines from a majority of the species investigated. These antibodies can be applied to flow cytometric studies of cytokine production by PBMCs from several veterinary species. Another five antibodies specific for IL-2, IL-6, GM-CSF and IFN-γ (two antibodies) cross-reacted weakly and with a variable number of animal species. These antibodies could in certain situations be useful in flow cytometry. In a number of cases the immunological cross-reactivities were confirmed by Western blot analyses. Overall, the results of this study will remedy some of the lack of species-specific anti-cytokine antibodies in veterinary research. [Copyright &y& Elsevier]
- Published
- 2002
- Full Text
- View/download PDF