1. TLR-2 expression and dysregulated human Treg/Th17 phenotype in Aspergillus flavus infected patients of chronic rhinosinusitis with nasal polyposis.
- Author
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Rai G, Das S, Ansari MA, Singh PK, Dar SA, Haque S, Gupta N, Sharma S, Ramachandran VG, Sharma S, Jain C, and Sharma S
- Subjects
- Adolescent, Adult, Aspergillus flavus, Chronic Disease, Female, Gene Expression Regulation, Host-Pathogen Interactions, Humans, Interleukin-10 immunology, Interleukin-17 immunology, Male, Middle Aged, RNA, Messenger immunology, Rhinitis immunology, Rhinitis microbiology, Sinusitis immunology, Sinusitis microbiology, Toll-Like Receptor 2 genetics, Young Adult, Aspergillosis immunology, Cytokines immunology, Nasal Polyps immunology, T-Lymphocytes, Regulatory immunology, Th17 Cells immunology, Toll-Like Receptor 2 immunology
- Abstract
Background: T helper (Th)17 and regulatory T (Treg) cells with toll-like receptor (TLR)-2 have been acknowledged to play a critical role in chronic rhinosinusitis with nasal polyposis (CRSwNP). However, its pathogenesis has been perplexed by conflicting reports on the role of Th17/Treg cells in patients of distinct ethnicities. We attempted to understand the role of Th responses induced during host defense against Aspergillus flavus., Results: The percentages of Th17 (CD4
+ CD161+ IL23R+ ) and Treg (CD4+ CD25+ FoxP3+ ) cell populations and various cytokine profiles in peripheral blood mononuclear cells (PBMCs) challenged by A. flavus antigens were characterized from 50 CRSwNP cases, before and after treatment, and in 50 healthy controls. TLR-2 expression was analyzed in tissues of cases and controls for disease co-relation. The major pathogen identified in our study was A. flavus by mycological investigations. A marked immune imbalance was noted with elevated Th17 and decreased Tregs in PBMCs of CRSwNP patients after A. flavus stimulation. Comparatively, interleukin (IL)-17 and IL-10 levels were increased, with low transforming growth factor (TGF)-β levels in A. flavus stimulated PBMC supernatants of patients. The mRNA expression of TLR-2 in polyps of CRSwNP patients indicated significant (p = 0.001) upregulation in comparison to the controls., Conclusions: Our data highlights the excessive expression of TLR-2 in nasal polyps contributing to the imbalance in Th17/Tregs population in patients. After therapy, recovery of Tregs cells indicates restoration and tissue homeostasis, though high circulating CD4+ CD161+ Th17 cells may continue to be a threat to patients predisposed to future recurrences. The constant exposure and tendency of A. flavus to colonize nasal cavities can lead to a Th17 driven airway inflammation. Dysregulated Th17 with TLR-2 promote resistance to treatment and progression to the chronicity of the disease.- Published
- 2020
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