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2 results on '"De Lima S"'

1. Cytokines and inflammatory mediators: Markers involved in interstitial damage to the pancreas in two dengue fever cases associated with acute pancreatitis.

Author

Alves FAV, Oliveira LLS, Salomão NG, Provance DW Jr, Basilio-de-Oliveira CA, Basílio-de-Oliveira R, Moragas LJ, de Carvalho JJ, Mohana-Borges R, Rabelo K, and Paes MV

Subjects
Adult, Apoptosis, Dengue virology, Female, Humans, Male, Middle Aged, Pancreatitis metabolism, Pancreatitis virology, Young Adult, Biomarkers metabolism, Cytokines metabolism, Dengue complications, Dengue Virus isolation & purification, Inflammation Mediators metabolism, Pancreatitis pathology
Abstract

Dengue viral (DENV) infections can lead to acute pancreatitis and associated tissue damage. This study examined the pancreas from two fatal cases of DENV for histopathological changes as well as for the detection of cytokines, and other inflammatory mediators. Tissue sections were prepared for examination by ultrastructural and histopathological techniques. Sections from the pancreas of non-infected individuals were prepared in parallel as a control. The presence of viral replication in macrophages was detected by co-staining for the proteins NS3 and CD68 by immunofluorescence. Immunohistochemistry was used to detect cells that expressed cytokines and inflammatory mediators to characterize the inflammatory response. Edema, acinar necrosis and fibrosis areas associated with a mononuclear infiltrate were found in infected tissues. The major site of virus replication appeared to be macrophages based on their exclusive presentation of the viral protein NS3. Pancreatic tissues from the infected individuals also displayed increased levels of high mobility group box-1, caspase-3, gelatinase B and tumor necrosis factor alpha compared to controls. The presence of virus replicating macrophages in the pancreas was associated with multiple changes in tissue structure that included elevated levels of cytokines and inflammatory markers that may differentiate acute pancreatitis due to DENV infections from other causes., Competing Interests: The authors have declared that no competing interests exist.

Published
2022
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2. Cytokines and inflammatory mediators: Markers involved in interstitial damage to the pancreas in two dengue fever cases associated with acute pancreatitis

Author

Felipe de Andrade Vieira Alves, Lucca de Lima S. Oliveira, Natália Gedeão Salomão, David William Provance, Carlos Alberto Basilio-de-Oliveira, Rodrigo Basílio-de-Oliveira, Leandro Junqueira Moragas, Jorge José de Carvalho, Ronaldo Mohana-Borges, Kíssila Rabelo, and Marciano Viana Paes

Subjects
Male, RNA viruses, Viral Diseases, Physiology, viruses, Apoptosis, Pathology and Laboratory Medicine, Biochemistry, Dengue Fever, Dengue, White Blood Cells, Medical Conditions, Animal Cells, Immune Physiology, Medicine and Health Sciences, Innate Immune System, Multidisciplinary, Middle Aged, Infectious Diseases, Medical Microbiology, Viral Pathogens, Viruses, Cytokines, Medicine, Female, Inflammation Mediators, Pathogens, Cellular Types, Anatomy, Research Article, Neglected Tropical Diseases, Adult, Immune Cells, Science, Immunology, Endocrine System, Gastroenterology and Hepatology, Microbiology, Young Adult, Exocrine Glands, Humans, Microbial Pathogens, Pancreas, Blood Cells, Flaviviruses, Macrophages, Organisms, Biology and Life Sciences, Proteins, Cell Biology, Dengue Virus, Molecular Development, Tropical Diseases, Fibrosis, Pancreatitis, Immune System, Collagens, Biomarkers, Developmental Biology
Abstract

Dengue viral (DENV) infections can lead to acute pancreatitis and associated tissue damage. This study examined the pancreas from two fatal cases of DENV for histopathological changes as well as for the detection of cytokines, and other inflammatory mediators. Tissue sections were prepared for examination by ultrastructural and histopathological techniques. Sections from the pancreas of non-infected individuals were prepared in parallel as a control. The presence of viral replication in macrophages was detected by co-staining for the proteins NS3 and CD68 by immunofluorescence. Immunohistochemistry was used to detect cells that expressed cytokines and inflammatory mediators to characterize the inflammatory response. Edema, acinar necrosis and fibrosis areas associated with a mononuclear infiltrate were found in infected tissues. The major site of virus replication appeared to be macrophages based on their exclusive presentation of the viral protein NS3. Pancreatic tissues from the infected individuals also displayed increased levels of high mobility group box-1, caspase-3, gelatinase B and tumor necrosis factor alpha compared to controls. The presence of virus replicating macrophages in the pancreas was associated with multiple changes in tissue structure that included elevated levels of cytokines and inflammatory markers that may differentiate acute pancreatitis due to DENV infections from other causes.

Published
2022

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