1. [Effect of Auricularia auricula polysaccharide on PBMCs proliferation and cytokine expression in vitro].
- Author
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Zuo JC, Zeng YQ, He XW, and Wang QX
- Subjects
- Cells, Cultured, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Humans, Interferon-gamma blood, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear metabolism, Polysaccharides isolation & purification, Tumor Necrosis Factor-alpha blood, Basidiomycota chemistry, Cell Proliferation drug effects, Cytokines blood, Leukocytes, Mononuclear drug effects, Polysaccharides pharmacology
- Abstract
Objective: To investigate the proliferation and cytokine expression of Auricularia auricula polysaccharide (AAP) on peripheral blood monnuclear cells (PBMCs) in vitro., Methods: AAP was obtained by water extraction and alcohol precipitation, and purified by DEAE-cellulose chromatogram. PBMCs were treated with AAP at different levels. The Cell Counting Kit-8 (CCK-8) was used to determine the proliferation of PBMCs. The concentration of TNF-alpha and TFN-gamma in supernatant were investigated by ELISA., Results: The three fractions of AAP could stimulate PBMCs proliferation at the range of 100--800 microg/ml, and AAP2 enhanced significantly the level of TNF-alpha and IFN-gamma in supernatant of PBMCs (P < 0.05, P < 0.01)., Conclusion: AAP is an immunomodulator and can enhance the immunomodulatory activity through TNF-alpha and TFN-gamma.
- Published
- 2008