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Micropropagation protocol for Diplocyclos palmatus through direct shoot induction from isolated cotyledon and leaf explants was established. Cotyledons were isolated from 2-week-old seedlings and cultured in the Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP, 0.5–2.0 mg L−1) alone or in combination with α-naphthalene acetic acid (NAA, 0.2–1.0 mg L−1) for shoot induction. The highest frequency of response (89%) and the mean number of shots (6.8) were observed in an MS medium supplemented with 1.5 mg L−1 BAP and 0.5 mg L−1 NAA. Leaves at various stages of development were isolated from in vitro grown shoots and cultured in an MS medium supplemented with different concentrations of BAP (0.5–2.5 mg L−1), Thidiazuron (TDZ, 0.5–2.5 mg L−1) alone and in combination with NAA (0.2 mg L−1) for shoot induction. The highest shoot induction frequency of 91% and shoot number of 7.8 shoots per explant were observed when 15-d-old- leaves were cultured in an MS medium enriched with 2.0 mg L−1 TDZ and 0.5 mg L−1 NAA after 45 d. The isolated cotyledon and leaf-derived shoots showed optimum rooting on ½ MS medium fortified with 4.0 mg L−1 indole-3-butyric acid (IBA). The rooted plants were transferred to the soil with 90% success. [ABSTRACT FROM AUTHOR] more...

In vitro regeneration of Melia azedarach L. was studied. Shoots were regenerated from calli initiated from leaflets of in vitro growing plants. The best medium for establishment of cultures was Murashige and Skoog (MS) medium with 4.44 μM benzylaminopurine (BAP) + 0.46 μM kinetin (KIN) + 16.29 μM adenine sulphate (ADE). Regenerated shoots were multiplied in MS + 0.44 μM BAP + 0.37 μM KIN + 3.26 μM ADE. Maximal rooting of 89 % was achieved by culture of regenerated shoots in MS + 12.26 μM indole-3-butyric acid for 3 d and subsequently in MS lacking growth regulators for 27 d. Rooted shoots were acclimatized and successfully transferred to soil. [ABSTRACT FROM AUTHOR] more...