1. A 'dry and wet hybrid' lithography technique for multilevel replication templates: Applications to microfluidic neuron culture and two-phase global mixing.
- Author
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Paul, Debjani, Saias, Laure, Pedinotti, Jean-Cedric, Chabert, Max, Magnifico, Sebastien, Pallandre, Antoine, De Lambert, Bertrand, Houdayer, Claude, Brugg, Bernard, Peyrin, Jean-Michel, and Viovy, Jean-Louis
- Subjects
MICROFLUIDICS ,LITHOGRAPHY ,NEURONS ,VISCOUS flow ,VISCOSITY solutions ,FLUID dynamics ,SURFACE tension ,CYTOLOGY - Abstract
A broad range of microfluidic applications, ranging from cell culture to protein crystallization, requires multilevel devices with different heights and feature sizes (from micrometers to millimeters). While state-of-the-art direct-writing techniques have been developed for creating complex three-dimensional shapes, replication molding from a multilevel template is still the preferred method for fast prototyping of microfluidic devices in the laboratory. Here, we report on a 'dry and wet hybrid' technique to fabricate multilevel replication molds by combining SU-8 lithography with a dry film resist (Ordyl). We show that the two lithography protocols are chemically compatible with each other. Finally, we demonstrate the hybrid technique in two different microfluidic applications: (1) a neuron culture device with compartmentalization of different elements of a neuron and (2) a two-phase (gas-liquid) global micromixer for fast mixing of a small amount of a viscous liquid into a larger volume of a less viscous liquid. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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