1. Evaluation of a multiplex PCR assay for detection of cytomegalovirus in stool samples from patients with ulcerative colitis.
- Author
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Nahar S, Iraha A, Hokama A, Uehara A, Parrott G, Ohira T, Kaida M, Kinjo T, Kinjo T, Hirata T, Kinjo N, and Fujita J
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Colitis, Ulcerative diagnosis, Colitis, Ulcerative immunology, Colonoscopy, Cytomegalovirus isolation & purification, Cytomegalovirus Infections immunology, Cytomegalovirus Infections virology, DNA, Viral isolation & purification, Feasibility Studies, Female, Humans, Immunocompetence, Immunocompromised Host, Male, Middle Aged, Opportunistic Infections immunology, Opportunistic Infections virology, Predictive Value of Tests, Prospective Studies, Reproducibility of Results, Young Adult, Colitis, Ulcerative drug therapy, Cytomegalovirus genetics, Cytomegalovirus Infections diagnosis, DNA, Viral genetics, Feces virology, Immunosuppressive Agents adverse effects, Multiplex Polymerase Chain Reaction, Opportunistic Infections diagnosis, Virology methods
- Abstract
Aim: To evaluate a multiplex PCR assay for the detection of bacterial and viral enteropathogens in stool samples from patients with ulcerative colitis (UC)., Methods: We prospectively analyzed 300 individuals, including immunocompetent patients, immunocompromised patients, and patients with UC. Stool samples were collected from the recto-sigmoid region of the colon by endoscopy. The samples were qualitatively analyzed for bacterial and viral enteropathogens with a multiplex PCR assay using a Seeplex(®) Kit. Additional clinical and laboratory data were collected from the medical records., Results: A multiplex PCR assay detected 397 pathogens (191 bacteria and 206 viruses) in 215 samples (71.7%). The most frequently detected bacteria were Escherichia coli H7, 85 (28.3%); followed by Aeromonas spp., 43 (14.3%); and Clostridium perfringens, 36 (12.0%) samples. The most prevalent viruses were Epstein-Barr virus (EBV), 90 (30.0%); followed by human herpes virus-6 (HHV-6), 53 (17.7%); and cytomegalovirus (CMV), 37 (12.3%) samples. The prevalence rate of CMV infection was significantly higher in the immunocompromised group than in the immunocompetent group (P < 0.01). CMV infection was more common in patients with UC (26/71; 36.6%) than in the immunocompetent patients excluding UC (6/188; 3.2%) (P < 0.01). CMV infection was more prevalent in UC active patients (25/58; 43.1%) than in UC inactive patients (1/13; 7.7%) (P < 0.05). Among 4 groups which defined by the UC activity and immunosuppressive drugs, the prevalence rate of CMV infection was highest in the UC active patients with immunosuppressive drugs (19/34; 55.8%). Epstein-Barr virus (EBV) infection was more common in the immunocompromised patients excluding UC (18/41; 43.9%) than in the immunocompetent patients excluding UC (47/188; 25.0%) (P < 0.05). The simultaneous presence of CMV and EBV and/or HHV6 in UC active patients (14/58; 24.1%) was greater than in immunocompromised patients excluding UC (5/41; 12.2%) (P < 0.05)., Conclusion: The multiplex PCR assay that was used to analyze the stool samples in this study may serve as a non-invasive approach that can be used to exclude the possibility of CMV infection in patients with active UC who are treated with immunosuppressive therapy.
- Published
- 2015
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