1. Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas
- Author
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Nina Himmerkus, Frank Melzner, Markus Bleich, Marian Y. Hu, and Kirti Ramesh
- Subjects
0106 biological sciences ,0301 basic medicine ,Sodium-Hydrogen Exchangers ,Physiology ,Bicarbonate ,Sodium ,Intracellular pH ,chemistry.chemical_element ,010603 evolutionary biology ,01 natural sciences ,Biochemistry ,Calcification ,Amiloride ,03 medical and health sciences ,chemistry.chemical_compound ,Endocrinology ,Calcification, Physiologic ,Animals ,Acid–base ,Crassostrea ,Mantle (mollusc) ,Carbonic Anhydrase Inhibitors ,Ecology, Evolution, Behavior and Systematics ,Ion transporter ,Original Paper ,Ion Transport ,biology ,Epithelial Cells ,Pacific oyster ,Hydrogen-Ion Concentration ,biology.organism_classification ,Cell biology ,Acetazolamide ,030104 developmental biology ,Ion transport proteins ,chemistry ,Animal Science and Zoology ,Cytophotometry ,Mollusc ,Low sodium ,Sodium Channel Blockers - Abstract
Shell formation and repair occurs under the control of mantle epithelial cells in bivalve molluscs. However, limited information is available on the precise acid–base regulatory machinery present within these cells, which are fundamental to calcification. Here, we isolate mantle epithelial cells from the Pacific oyster, Crassostrea gigas and utilise live cell imaging in combination with the fluorescent dye, BCECF-AM to study intracellular pH (pHi) regulation. To elucidate the involvement of various ion transport mechanisms, modified seawater solutions (low sodium, low bicarbonate) and specific inhibitors for acid–base proteins were used. Diminished pH recovery in the absence of Na+ and under inhibition of sodium/hydrogen exchangers (NHEs) implicate the involvement of a sodium dependent cellular proton extrusion mechanism. In addition, pH recovery was reduced under inhibition of carbonic anhydrases. These data provide the foundation for a better understanding of acid–base regulation underlying the physiology of calcification in bivalves. Electronic supplementary material The online version of this article (10.1007/s00360-020-01303-3) contains supplementary material, which is available to authorized users.
- Published
- 2019