1. Real-time fluorescence and deformability cytometry.
- Author
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Rosendahl P, Plak K, Jacobi A, Kraeter M, Toepfner N, Otto O, Herold C, Winzi M, Herbig M, Ge Y, Girardo S, Wagner K, Baum B, and Guck J
- Subjects
- HeLa Cells, Hematopoietic Stem Cells physiology, Humans, Lasers, Microfluidic Analytical Techniques instrumentation, Microfluidic Analytical Techniques methods, RNA Interference, Reticulocytes, Single-Cell Analysis methods, Cytophotometry methods, Optical Imaging methods
- Abstract
The throughput of cell mechanical characterization has recently approached that of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we developed an approach that combines real-time 1D-imaging fluorescence and deformability cytometry in one instrument (RT-FDC), thus opening many new research avenues. We demonstrated its utility by using subcellular fluorescence localization to identify mitotic cells and test for mechanical changes in those cells in an RNA interference screen.
- Published
- 2018
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