1. Role of accessory cells in the induction of a secondary cytotoxic response to Moloney murine sarcoma virus-induced tumors.
- Author
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Biasi G, Saggin L, Dazzi F, De Rossi A, and Collavo D
- Subjects
- Animals, Antigens, Viral immunology, Epitopes, Histocompatibility Antigens Class II immunology, Immunity, Cellular, Mice, Mice, Inbred A, Moloney murine leukemia virus immunology, Sarcoma, Experimental etiology, Spleen cytology, Spleen immunology, Cytotoxicity, Immunologic, Sarcoma, Experimental immunology
- Abstract
The role of Ia-positive accessory cells in the generation of a secondary cytotoxic response to tumor-associated antigens induced by Moloney murine sarcoma virus (M-MSV) was evaluated. Spleen cells from M-MSV-immune A.TL mice, depleted of accessory cells by anti-Iak serum plus C treatment and stimulated in secondary mixed leukocyte tumor cell culture (MLTC) with syngeneic Ia-negative A6ATL Moloney leukemic cells, failed to generate virus-specific cytotoxic T lymphocytes (CTL). CTL generation in Ia-depleted MLTC may be reconstituted by the addition of nonimmune Ia-positive spleen or peritoneal cells obtained not only from syngeneic A.TL but also from I-incompatible A.TH mice. This lack of restriction observed in accessory cell function is explained in terms of a nonspecific mechanism of CTL triggering mediated by soluble factors. In fact, IL 2 as well as supernatants obtained from I region-incompatible cultures consisting of M-MSV-immune, Ia-depleted A.TL spleen cells and A.TH Ia-positive cells, reconstituted secondary virus-specific CTL generation.
- Published
- 1983