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Your search keyword '"Interleukin-12 Subunit p40 drug effects"' showing total 2 results
Start Over Descriptor "Interleukin-12 Subunit p40 drug effects" Topic dendritic cells
2 results on '"Interleukin-12 Subunit p40 drug effects"'

1. The effect of caffeic acid phenethyl ester on the functions of human monocyte-derived dendritic cells.

Author

Wang LC, Lin YL, Liang YC, Yang YH, Lee JH, Yu HH, Wu WM, and Chiang BL

Subjects
Allergens immunology, Animals, Chemokine CXCL10 antagonists & inhibitors, Chemokine CXCL10 immunology, Chemokine CXCL10 metabolism, Dendritic Cells immunology, Humans, Hypersensitivity metabolism, I-kappa B Kinase antagonists & inhibitors, I-kappa B Kinase immunology, I-kappa B Kinase metabolism, Interleukin-10 antagonists & inhibitors, Interleukin-10 immunology, Interleukin-10 metabolism, Interleukin-12 antagonists & inhibitors, Interleukin-12 immunology, Interleukin-12 metabolism, Interleukin-12 Subunit p40 antagonists & inhibitors, Interleukin-12 Subunit p40 drug effects, Interleukin-12 Subunit p40 immunology, Interleukin-12 Subunit p40 metabolism, Lipopolysaccharides pharmacology, Mitogen-Activated Protein Kinase Kinases immunology, Mitogen-Activated Protein Kinase Kinases metabolism, NF-kappa B antagonists & inhibitors, NF-kappa B immunology, NF-kappa B metabolism, Phenylethyl Alcohol pharmacology, Phosphorylation drug effects, Phosphorylation immunology, Pyroglyphidae immunology, Signal Transduction drug effects, Signal Transduction immunology, T-Lymphocytes drug effects, T-Lymphocytes metabolism, Caffeic Acids pharmacology, Dendritic Cells drug effects, Hypersensitivity immunology, Phenylethyl Alcohol analogs & derivatives, Propolis pharmacology, T-Lymphocytes immunology
Abstract

Background: Propolis, an ancient herbal medicine, has been reported the beneficial effect both in asthma patients and murine model of asthma, but the mechanism was not clearly understood. In this study, the effect of caffeic acid phenethyl ester (CAPE), the most extensively studied components in propolis, on the functions of human monocyte-derived dendritic cells (MoDCs) was investigated., Results: CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract. CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract. In contrast, the upregulation of costimulatory molecules in mature MoDCs was not suppressed by CAPE. Further, the antigen presenting ability of DCs was not inhibited by CAPE. CAPE inhibited IkappaBalpha phosphorylation and NF-kappaB activation but not mitogen-activated protein kinase (MAPK) family phosphorylation in human MoDCs., Conclusion: These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway. This study provided a new insight into the mechanism of CAPE in immune response and the rationale for propolis in the treatment of asthma and other allergic disorders.

Published
2009
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2. Armillariella mellea induces maturation of human dendritic cells without induction of cytokine expression.

Author

Kim SK, Im J, Yun CH, Son JY, Son CG, Park DK, and Han SH

Subjects
Antigens, CD drug effects, Antigens, CD metabolism, Cell Differentiation drug effects, Cytokines metabolism, Dendritic Cells metabolism, Dextrans, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fluorescein-5-isothiocyanate analogs & derivatives, Gene Expression Regulation drug effects, Humans, Interleukin-10 metabolism, Interleukin-12 Subunit p40 drug effects, Interleukin-12 Subunit p40 metabolism, Lipopolysaccharides pharmacology, Tumor Necrosis Factor-alpha drug effects, Tumor Necrosis Factor-alpha metabolism, Agaricales chemistry, Cytokines drug effects, Dendritic Cells drug effects
Abstract

Ethnopharmacological Relevance: Armillariella mellea, an edible and medicinal mushroom possessing immuno-modulating potential, has been frequently used for the treatment of infectious diseases or cancers., Aim of the Study: In order to elucidate immune-regulatory mechanisms of Armillariella mellea, we investigated the effect of water-soluble components from Armillariella mellea (AME) on the regulation of human dendritic cell (DC) maturation and activation., Materials and Methods: Immature DCs (iDCs) were prepared by differentiating human peripheral blood CD14-positive cells with GM-CSF and IL-4. Then, iDCs were treated with AME at 2-20 microg/ml for 48 h and subjected to flow cytometry to analyze the expression of DC markers. Dextran-FITC uptake assay and enzyme-linked immunosorbent assay were performed to examine the endocytic capacity of AME-stimulated DC and their production of cytokines, respectively., Results: iDCs stimulated with AME showed representative features during DC maturation such as up-regulated expression of CD80, CD83, CD86, both MHC class I and II molecules, and CD205, with a simultaneous decrease in the expression of CD206 and the endocytic capacity. Interestingly, AME was not able to induce the production of TNF-alpha, IL-12p40, or IL-10, whereas lipopolysaccharides induced a substantial increase of all of the cytokines., Conclusion: Armillariella mellea induces maturation of human DCs through a unique mechanism without inducing cytokine expression.

Published
2008
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