1. Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization.
- Author
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Moon HG, Kim SJ, Jeong JJ, Han SS, Jarjour NN, Lee H, Abboud-Werner SL, Chung S, Choi HS, Natarajan V, Ackerman SJ, Christman JW, and Park GY
- Subjects
- Animals, Cell Line, Cell Movement immunology, Dendritic Cells classification, Epithelial Cells cytology, Epithelial Cells immunology, Humans, Immunity, Innate immunology, Immunoglobulin E immunology, Interferon Regulatory Factors immunology, Lymph Nodes cytology, Mice, Mice, Inbred C57BL, Mice, Transgenic, RAW 264.7 Cells, Receptors, Granulocyte-Macrophage Colony-Stimulating Factor antagonists & inhibitors, Receptors, Granulocyte-Macrophage Colony-Stimulating Factor metabolism, Th2 Cells immunology, Up-Regulation immunology, Allergens immunology, Dendritic Cells immunology, Macrophage Colony-Stimulating Factor immunology, Receptors, CCR7 biosynthesis, Respiratory Mucosa cytology, Respiratory Mucosa immunology
- Abstract
Airway epithelial cells (AECs) secrete innate immune cytokines that regulate adaptive immune effector cells. In allergen-sensitized humans and mice, the airway and alveolar microenvironment is enriched with colony stimulating factor-1 (CSF1) in response to allergen exposure. In this study we found that AEC-derived CSF1 had a critical role in the production of allergen reactive-IgE production. Furthermore, spatiotemporally secreted CSF1 regulated the recruitment of alveolar dendritic cells (DCs) and enhanced the migration of conventional DC2s (cDC2s) to the draining lymph node in an interferon regulatory factor 4 (IRF4)-dependent manner. CSF1 selectively upregulated the expression of the chemokine receptor CCR7 on the CSF1R
+ cDC2, but not the cDC1, population in response to allergen stimuli. Our data describe the functional specification of CSF1-dependent DC subsets that link the innate and adaptive immune responses in T helper 2 (Th2) cell-mediated allergic lung inflammation., (Copyright © 2018 Elsevier Inc. All rights reserved.)- Published
- 2018
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