1. Effect of S 2 O 3 2- -S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects.
- Author
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Liu H, Zeng W, Li J, Zhan M, Fan Z, and Peng Y
- Subjects
- Bacteria, Isotopes analysis, Nitrogen analysis, Nitrogen Dioxide analysis, Oxidation-Reduction, Sulfur, Wastewater analysis, Bioreactors, Denitrification
- Abstract
Anaerobic ammonia oxidation (Anammox) coupling sulfur autotrophic denitrification is an effective method for the advanced nitrogen removal from the wastewater with limited carbon source. The influence of S
2 O3 -S addition on Anammox coupling sulfur autotrophic denitrification was investigated by adding different concentrations of S2- -S addition on Anammox coupling sulfur autotrophic denitrification was investigated by adding different concentrations of S2 -S (0, 39, 78, 156 and 312 mg/L) to the Anammox system. The contribution of sulfur autotrophic denitrification and Anammox to nitrogen removal at S3 2- -S (0, 39, 78, 156 and 312 mg/L) to the Anammox system. The contribution of sulfur autotrophic denitrification and Anammox to nitrogen removal at S2 O3 -S concentrations up to 312 mg/L, and only sulfur autotrophic denitrification occurred. The isotopic effects of NO2- -S concentrations of 156 mg/L was 75% ∼83% and 17%∼25%, respectively, and the mixed system achieved completely nitrogen removal. However, Anammox bioactivity was completely inhibited at S2 O3 2- -S concentrations up to 312 mg/L, and only sulfur autotrophic denitrification occurred. The isotopic effects of NO2 - -N (δ15 NNO2 and δ18 ONO2 ) during Anammox coupling sulfur autotrophic denitrification showed a gradual decrease trend with the increase of S3 - -N (δ15 NNO3 and δ18 ONO3 ) during Anammox coupling sulfur autotrophic denitrification showed a gradual decrease trend with the increase of S2 O3 2- -S addition. The ratios of δ15 NNO2 :δ18 ONO2 and δ15 NNO3 :δ18 ONO3 was maintained at 1.30-2.41 and 1.36-2.52, respectively, which revealed that Anammox was dominant nitrogen removal pathway at S2 -driven autotrophic denitrification and weakened the Anammox, leading to a gradually decreasing trend of the proportion of Candidatus Brocadia as Anammox bacteria from the initial 27% to 4% (S3 2- -S of 156 mg/L). Yet Norank-f-Hydrogenophilaceae (more than 50%) and Thiobacillus (54%) as functional bacteria of autotrophic denitrification obviously increased. The appropriate amount of S2 O3 -S addition promoted the performance of Anammox coupling sulfur autotrophic denitrification achieved completely nitrogen removal.2- -S. The S2 O3 2- -S addition enhanced the S2 O3 2- -driven autotrophic denitrification and weakened the Anammox, leading to a gradually decreasing trend of the proportion of Candidatus Brocadia as Anammox bacteria from the initial 27% to 4% (S2 O3 2- -S of 156 mg/L). Yet Norank-f-Hydrogenophilaceae (more than 50%) and Thiobacillus (54%) as functional bacteria of autotrophic denitrification obviously increased. The appropriate amount of S2 O3 2- -S addition promoted the performance of Anammox coupling sulfur autotrophic denitrification achieved completely nitrogen removal., (Copyright © 2022. Published by Elsevier Ltd.)- Published
- 2022
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