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Aim: The aim of the study was to determine the concentration of fluoride in natural mineral waters marketed in Spain in order to prevent tooth decay without the risk of causing dental fluorosis, Design: Descriptive and cross-sectional study during 2012., Location: Natural mineral waters marketed in Spain., Participants: Three bottles with different bottling dates of 109 natural mineral waters (97 Spanish and 12 imported brands)., Main Measures: Determination of fluoride by ion chromatography, Results: Median fluoride concentrations of the natural mineral waters bottled in Spain was 0.22 (range 0.00-4.16; interquartile range:0.37). Most samples (61 brands, 62%) contained less than 0.3mg/L. There are 19 Spanish brands with more than 0.6 mg/L. The median level in imported brands was 0.35 (range 0.10-1.21; interquartile range: 0.23). Only 28 of the 109 brands examined (25.6%) specified the fluoride content on the label. Good correlation was observed between the concentrations indicated and those determined., Conclusions: Fluoride concentrations in natural mineral waters showed high variation. Given the growing consumption of natural mineral waters in Spain, this type of information is important to make proper use of fluoride in the primary prevention of dental caries., (Copyright © 2014 Elsevier España, S.L.U. All rights reserved.)