1. The Potential Application of Human Gingival Fibroblast-Conditioned Media in Pulp Regeneration: An In Vitro Study.
- Author
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Vu HT, Yoon JY, Park JH, Lee HH, Dashnyam K, Kim HW, Lee JH, Shin JS, and Kim JB
- Subjects
- Humans, Alkaline Phosphatase metabolism, Fibroblasts metabolism, Regeneration, Gingiva cytology, Gingiva metabolism, Culture Media, Conditioned chemistry, Culture Media, Conditioned pharmacology, Dental Pulp drug effects, Dental Pulp metabolism, Hydrogen Peroxide, Tissue Engineering methods
- Abstract
Regenerative endodontic treatment based on tissue engineering has recently gained interest in contemporary restorative dentistry. However, low survival rates and poor potential differentiation of stem cells could undermine the success rate of pulp regenerative therapy. Human gingival fibroblast-conditioned medium (hGF-CM) has been considered a potential therapy for tissue regeneration due to its stability in maintaining multiple factors essential for tissue regeneration compared to live cell transplantation. This study aimed to investigate the potency of hGF-CM on stem cells from human dental pulp (DPSC) in pulp regeneration. A series of experiments confirmed that hGF-CM contributes to a significant increase in proliferation, migration capability, and cell viability of DPSC after H
2 O2 exposure. Moreover, it has been proved to facilitate the odontogenic differentiation of DPSC via qRT-PCR, ALP (alkaline phosphatase), and ARS (Alizarin Red S) staining. It has been discovered that such highly upregulated odontogenesis is related to certain types of ECM proteins (collagen and laminin) from hGF-CM via proteomics. In addition, it is found that the ERK pathway is a key mechanism via inhibition assay based on RNA-seq result. These findings demonstrate that hGF-CM could be beneficial biomolecules for pulp regeneration., Competing Interests: The authors declare no conflict of interest.- Published
- 2022
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