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Start Over Author "Frozoni M" Topic dentinogenesis
3 results on '"Frozoni M"'

1. Contribution of Bone Marrow-derived Cells to Reparative Dentinogenesis Using Bone Marrow Transplantation Model.

Author

Frozoni M, Marques MR, Hamasaki SK, Mohara NT, de Jesus Soares A, and Zaia AA

Subjects
Animals, Bone Marrow, Bone Marrow Cells, Dental Pulp, France, Mice, Mice, Inbred C57BL, Bone Marrow Transplantation, Dentinogenesis
Abstract

Introduction: The aim of this study was to analyze the contribution of bone marrow-derived cells (BMDCs) to reparative dentinogenesis using bone marrow transplantation (BMT) and pulp capping as an in vivo model., Methods: A chimeric mouse model was created through the injection of BMDCs expressing green fluorescent protein (GFP+ BMDCs) from C57BL/6 GFP+ transgenic donor mice into irradiated C57BL/6 wild-type recipient mice (GFP- mice). These GFP- chimeric mice (containing transplanted GFP+ BMDCs) were subjected to microscopic pulp exposure and capping with white mineral trioxide aggregate (n = 18) or Biodentine (Septodont, St Maur-des-Fossés, France) (n = 18) in the maxillary first molar. Maxillary arches from GFP- chimeric mice (with the capped tooth) were isolated and histologically processed 5 (n = 9) and 7 (n = 9) weeks after BMT. Confocal laser microscopy and immunohistochemical analysis were performed to assess the presence of GFP+ BMDCs and the expression of dentin sialoprotein, an odontoblast marker, for those cells contributing to reparative dentinogenesis in the dental pulp., Results: Confocal laser microscopic analyses evidenced the presence of GFP+ BMDCs in close association with reparative dentin synthesized at the site of pulp exposure in GFP- mice 5 and 7 weeks after BMT. Immunohistochemical analysis revealed that GFP+ BMDCs in close association with reparative dentin expressed DSP, suggesting the contribution of nonresident GFP+ BMDCs to reparative dentinogenesis., Conclusions: These data suggest the presence of nonresident BMDCs in reparative dentinogenesis and its contribution to dental pulp regeneration in the pulp healing process., (Copyright © 2019 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)

Published
2020
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2. Analysis of the contribution of nonresident progenitor cells and hematopoietic cells to reparative dentinogenesis using parabiosis model in mice.

Author

Frozoni M, Zaia AA, Line SR, and Mina M

Subjects
Acid Phosphatase analysis, Aluminum Compounds therapeutic use, Animals, Biomarkers analysis, Calcium Compounds therapeutic use, Composite Resins chemistry, Cross Circulation methods, Dental Materials chemistry, Dental Pulp Capping methods, Dental Pulp Exposure pathology, Dental Pulp Exposure therapy, Dentin, Secondary physiology, Drug Combinations, Flow Cytometry, Fluorescent Dyes, Green Fluorescent Proteins, Isoenzymes analysis, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, Fluorescence, Models, Animal, Molar pathology, Molar physiopathology, Odontoblasts pathology, Oxides therapeutic use, Pulp Capping and Pulpectomy Agents therapeutic use, Resin Cements chemistry, Silicates therapeutic use, Silicon Dioxide chemistry, Tartrate-Resistant Acid Phosphatase, Zirconium chemistry, Dentinogenesis physiology, Hematopoietic Stem Cells physiology, Parabiosis methods, Stem Cells physiology
Abstract

Introduction: The aim of this study was to analyze the contribution of nonresident progenitor/stem cells and hematopoietic cells to reparative dentinogenesis., Methods: Parabiosis was established between C57BL/6-TgN(ACTbEGFP)10sb/J transgenic mice (GFP+) and C57BL/6 wild-type mice (GFP-) to ensure blood cross-circulation between animals. Reparative dentinogenesis was stimulated by pulp exposures and capping on the first maxillary molar in the GFP- mice. Histologic sections of injured molars from GFP- mice were analyzed by epifluorescence microscopy to examine the contributions of GFP+ cells (nonresident progenitor cells and hematopoietic cells originating from GFP+ mice) to reparative dentinogenesis., Results: GFP+ cells were detected in close association with reparative dentin formed at the site of pulp exposure in the maxillary first molars of the GFP- mice., Conclusions: The present study suggests the participation of the nonresident progenitor cells and hematopoietic cells in reparative dentinogenesis., (Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)

Published
2012
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3. A feasibility study for the analysis of reparative dentinogenesis in pOBCol3.6GFPtpz transgenic mice

Author

Frozoni, M., Balic, A., Sagomonyants, K., Zaia, A. A., Line, S. R. P., and Mina, M.

Subjects
Extracellular Matrix Proteins, Wound Healing, Odontoblasts, Sialoglycoproteins, Silicates, Green Fluorescent Proteins, Mice, Transgenic, Oxides, Calcium Compounds, Dentinogenesis, Dentin, Secondary, Phosphoproteins, Models, Biological, Article, Dental Pulp Capping, Resin Cements, Drug Combinations, Mice, Gene Expression Regulation, Dentin-Bonding Agents, Animals, Feasibility Studies, Dental Pulp Exposure, Aluminum Compounds, Pulp Capping and Pulpectomy Agents
Abstract

To examine the feasibility of using the pOBCol3.6GFPtpz [3.6-green fluorescent protein (GFP)] transgenic mice as an in vivo model for studying the biological sequence of events during pulp healing and reparative dentinogenesis.Pulp exposures were created in the first maxillary molar of 12-16-week-old 3.6-GFP transgenic mice with CD1 and C57/Bl6 genetic background. Direct pulp capping on exposed teeth was performed using mineral trioxide aggregate followed by restoration with a light-cured adhesive system (AS) and composite resin. In control teeth, the AS was placed in direct contact with the pulp. Animals were euthanized at various time points after pulp exposure and capping. The maxillary arch was isolated, fixed and processed for histological and epifluorescence analysis to examine reparative dentinogenesis.Analysis of teeth immediately after pulp exposure revealed absence of odontoblasts expressing 3.6-GFP at the injury site. Evidence of reparative dentinogenesis was apparent at 4 weeks in 3.6-GFP mice in CD1 background and at 8 weeks in 3.6-GFP mice with C57/Bl6 background. The reparative dentine with both groups contained newly formed atubular-mineralized tissue resembling a dentine bridge and/or osteodentine that was lined by cells expressing 3.6-GFP as well as 3.6-GFP expressing cells embedded within the atubular matrix.This study was conducted in a few animals and did not allow statistical analysis. The results revealed that the 3.6-GFP transgenic animals provide a unique model for direct analysis of cellular and molecular mechanisms of pulp repair and tertiary dentinogenesis in vivo. The study also shows the effects of the capping material and the genetic background of the mice in the sequence and timing of reparative dentinogenesis.

Published
2012

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