21 results on '"Fujita, Hideki"'
Search Results
2. Increased syndecan-4 expression in sera and skin of patients with atopic dermatitis.
- Author
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Nakao M, Sugaya M, Takahashi N, Otobe S, Nakajima R, Oka T, Kabasawa M, Suga H, Morimura S, Miyagaki T, Fujita H, Asano Y, and Sato S
- Subjects
- Adult, Dermatitis, Atopic blood, Dermatitis, Atopic drug therapy, Endothelial Cells metabolism, Epidermal Cells, Epidermis pathology, Female, Healthy Volunteers, Humans, Male, Middle Aged, RNA, Messenger metabolism, Severity of Illness Index, Syndecan-4 blood, Young Adult, Dermatitis, Atopic metabolism, Epidermis metabolism, Pruritus metabolism, Syndecan-4 metabolism
- Abstract
Syndecan-4 (SDC-4) is a cell surface proteoglycan, which participates in signaling during cell adhesion, migration, proliferation, endocytosis, and mechanotransduction, and is expressed on various cells, including endothelial cells, epithelial cells, T cells, and eosinophils. Emerging evidences have suggested that SDC-4 might contribute to Th2-driven allergic immune responses. Here, we examined the role of SDC-4 in patients with atopic dermatitis (AD). Serum SDC-4 levels in AD patients were significantly higher than in healthy individuals, and they increased according to the disease severity. Importantly, they positively correlated with Eczema Area and Severity Index and itch visual analogue scale scores. Furthermore, serum SDC-4 levels decreased after treatment. We also analyzed SDC-4 expression in AD lesional skin. SDC-4 mRNA levels in AD skin were significantly higher than those of normal skin. Immunohistochemical staining revealed that SDC-4 was highly expressed in the epidermis and endothelial cells in AD lesional skin. Taken together, our study has demonstrated that SDC-4 expression was increased in sera and skin of AD patients, suggesting that SDC-4 may contribute to the development of AD.
- Published
- 2016
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3. CCR4 is expressed on infiltrating cells in lesional skin of early mycosis fungoides and atopic dermatitis.
- Author
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Sugaya M, Morimura S, Suga H, Kawaguchi M, Miyagaki T, Ohmatsu H, Fujita H, and Sato S
- Subjects
- Dermatitis, Atopic blood, Disease Progression, Humans, Immunohistochemistry, L-Lactate Dehydrogenase blood, Mycosis Fungoides blood, Psoriasis blood, Reagent Kits, Diagnostic, Sezary Syndrome blood, Skin chemistry, Dermatitis, Atopic metabolism, Mycosis Fungoides chemistry, Psoriasis metabolism, Receptors, CCR4 analysis, Sezary Syndrome chemistry
- Abstract
CCR4 is expressed on tumor cells of mycosis fungoides (MF) and Sézary syndrome (SS). In MF, most infiltrating cells in patches and plaques express CXCR3, while tumor cells express CCR4 in advanced stages. Poteligeo Test IHC (CCR4 staining kit) is a newly developed staining kit that can examine the presence of CCR4 expressed on tumor cells of adult T-cell leukemia/lymphoma, peripheral T-cell lymphoma and cutaneous T-cell lymphoma before treatment of anti-CCR4 antibody using paraffin-embedded samples. In this study, we analyzed CCR4 expression in lesional skin of MF, SS, atopic dermatitis (AD) and psoriasis with this new kit. CCR4 was expressed on infiltrating cells in lesional skin of patch, plaque, tumor MF and SS, and the number of positive cells increased as the disease progressed. Immunohistochemistry with frozen sections also showed some positive cells scattered in the dermis, although the quality was not high enough to quantify positive cells. There were significant positive correlations between CCR4(+) cells and serum lactate dehydrogenase levels. Interestingly, CCR4(+) cells were also detected in AD skin, whose number was larger than that in psoriatic skin. Previous studies showed only scattered CCR4(+) cells in skin samples by standard immunohistochemical staining. The new, sensitive CCR4 staining kit has revealed that CCR4 is expressed on infiltrating cells in lesional skin of early MF and AD as well as advanced MF and SS. These cells can be therapeutic targets for patients who are resistant to standard treatments., (© 2015 Japanese Dermatological Association.)
- Published
- 2015
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4. Serum autotaxin levels correlate with pruritus in patients with atopic dermatitis.
- Author
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Nakao M, Sugaya M, Suga H, Kawaguchi M, Morimura S, Kai H, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, and Sato S
- Subjects
- Adolescent, Adult, Case-Control Studies, Child, Dermatitis, Atopic complications, Female, Humans, Hydrolysis, Male, Middle Aged, Pruritus complications, Signal Transduction, Treatment Outcome, Young Adult, Dermatitis, Atopic blood, Gene Expression Regulation, Phosphoric Diester Hydrolases blood, Pruritus blood
- Published
- 2014
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5. Variations in serum TARC and I-TAC levels reflect minor changes in disease activity and pruritus in atopic dermatitis.
- Author
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Kimura T, Sugaya M, Suga H, Morimura S, Miyamoto A, Kai H, Kagami S, Yanaba K, Fujita H, Asano Y, Tada Y, Kadono T, and Sato S
- Subjects
- Adult, Biomarkers blood, Dermatitis, Atopic diagnosis, Female, Humans, Male, Pruritus diagnosis, Severity of Illness Index, Time Factors, Young Adult, Chemokine CCL17 blood, Chemokine CXCL11 blood, Dermatitis, Atopic blood, Pruritus blood
- Published
- 2014
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6. Serum visfatin levels in patients with atopic dermatitis and cutaneous T-cell lymphoma.
- Author
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Suga H, Sugaya M, Miyagaki T, Kawaguchi M, Morimura S, Kai H, Kagami S, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, and Sato S
- Subjects
- Adipose Tissue enzymology, Adult, Aged, Blood Cell Count, Body Mass Index, Case-Control Studies, Chemokine CCL11 blood, Chemokine CCL26, Chemokines, CC blood, Dermatitis, Atopic blood, Eosinophils, Female, Humans, Male, Middle Aged, Mycosis Fungoides pathology, Mycosis Fungoides therapy, Pruritus blood, Severity of Illness Index, Sezary Syndrome pathology, Sezary Syndrome therapy, Skin enzymology, Skin Neoplasms pathology, Skin Neoplasms therapy, Young Adult, Cytokines blood, Dermatitis, Atopic enzymology, Mycosis Fungoides metabolism, Nicotinamide Phosphoribosyltransferase blood, Sezary Syndrome metabolism, Skin Neoplasms metabolism
- Abstract
Visfatin, a novel adipocytokine, is related with chronic inflammatory diseases, especially those characterized by T helper (Th)1-type immune responses. In this study, we examined serum visfatin levels in patients with atopic dermatitis (AD) or cutaneous T-cell lymphoma (CTCL), both of which are Th2-dominant diseases. Serum visfatin levels in patients with AD or advanced stage CTCL were significantly elevated compared to healthy controls. In CTCL patients, serum visfatin levels significantly decreased after treatment. Serum visfatin levels correlated with eosinophil counts in AD patients, whereas they correlated with the visual analogue scale itch scores and serum C-C motif ligand (CCL) 11 and CCL26 levels in CTCL patients. Visfatin expression by adipose tissue in lesional skin of AD and advanced stage CTCL was enhanced compared to that of healthy controls. These results suggest that visfatin may also be important in the development of Th2-dominant diseases as well as in Th1-type diseases.
- Published
- 2013
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7. Serum gastrin-releasing peptide levels correlate with pruritus in patients with atopic dermatitis.
- Author
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Kagami S, Sugaya M, Suga H, Morimura S, Kai H, Ohmatsu H, Fujita H, Tsunemi Y, and Sato S
- Subjects
- Adult, Female, Humans, Male, Middle Aged, Young Adult, Dermatitis, Atopic blood, Dermatitis, Atopic immunology, Gastrin-Releasing Peptide blood, Pruritus blood, Pruritus immunology
- Published
- 2013
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8. Lesional dendritic cells in patients with chronic atopic dermatitis and psoriasis exhibit parallel ability to activate T-cell subsets.
- Author
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Fujita H, Shemer A, Suárez-Fariñas M, Johnson-Huang LM, Tintle S, Cardinale I, Fuentes-Duculan J, Novitskaya I, Carucci JA, Krueger JG, and Guttman-Yassky E
- Subjects
- Chemokines immunology, Chemokines metabolism, Humans, Lymphocyte Activation immunology, Skin pathology, Th1 Cells immunology, Th17 Cells immunology, Th2 Cells immunology, Dendritic Cells immunology, Dermatitis, Atopic immunology, Psoriasis immunology, Skin immunology, T-Lymphocyte Subsets immunology
- Abstract
Background: Atopic dermatitis (AD) and psoriasis represent polar immune diseases. AD is a T(H)2/T(H)22-dominant disease, whereas psoriasis is considered a T(H)1/T(H)17 disease. Local immune deviation is suggested to be regulated by dendritic cell (DC)-induced T-cell polarization and recruitment of specific T-cell subsets by chemokines. Although the role of chemokines is well documented, the actual contribution of DCs to activate polar T-cell subsets in human subjects is still a matter of speculation., Objective: We sought to elucidate the significance of each cutaneous DC subset in disease-specific T-cell immune deviation., Methods: We performed a comprehensive analysis of major cutaneous resident (Langerhans cells and blood dendritic cell antigen 1-positive dermal DCs) and inflammatory (inflammatory dendritic epidermal cells and blood dendritic cell antigen 1-negative dermal DCs) DC subsets directly isolated from the lesional skin of patients with AD and those with psoriasis., Results: The ability of each DC subset to expand T(H)1, T(H)2, T(H)17, and T(H)22 subsets was similar between the 2 diseases, despite the association of both with accumulation of resident and inflammatory DCs. We also confirmed differential upregulation of chemokine expression in patients with AD (CCL17, CCL18, and CCL22) and psoriasis (CXCL1, IL-8, and CCL20). The expression of CCL17 and CCL22 was higher in Langerhans cells from patients with AD than from patients with psoriasis, whereas the opposite was observed for CXCL9 and CXCL10., Conclusion: Our results suggest that DC polarity does not directly drive differential T-cell subset responses. Alternatively, disease-specific chemokines might recruit specific memory T-cell subsets into the skin, which in turn might be activated and expanded by DCs at the site of inflammation, maintaining differential immune polarity in these diseases., (Copyright © 2011 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.)
- Published
- 2011
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9. Reversal of atopic dermatitis with narrow-band UVB phototherapy and biomarkers for therapeutic response.
- Author
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Tintle S, Shemer A, Suárez-Fariñas M, Fujita H, Gilleaudeau P, Sullivan-Whalen M, Johnson-Huang L, Chiricozzi A, Cardinale I, Duan S, Bowcock A, Krueger JG, and Guttman-Yassky E
- Subjects
- Adult, Biomarkers, Chronic Disease, Dermatitis, Atopic immunology, Dermatitis, Atopic pathology, Female, Histocompatibility Antigens Class I immunology, Humans, Inflammation therapy, Male, Middle Aged, Skin immunology, Skin pathology, Th1 Cells immunology, Th2 Cells immunology, Treatment Outcome, Young Adult, Dermatitis, Atopic therapy, Ultraviolet Therapy
- Abstract
Background: Atopic dermatitis (AD) is a common inflammatory skin disease exhibiting a predominantly T(H)2/"T22" immune activation and a defective epidermal barrier. Narrow-band UVB (NB-UVB) is considered an efficient treatment for moderate-to-severe AD. In patients with psoriasis, NB-UVB has been found to suppress T(H)1/T(H)17 polarization, with subsequent reversal of epidermal hyperplasia. The immunomodulatory effects of this treatment are largely unknown in patients with AD., Objective: We sought to evaluate the effects of NB-UVB on immune and barrier abnormalities in patients with AD, aiming to establish reversibility of disease and biomarkers of therapeutic response., Methods: Twelve patients with moderate-to-severe chronic AD received NB-UVB phototherapy 3 times weekly for up to 12 weeks. Lesional and nonlesional skin biopsy specimens were obtained before and after treatment and evaluated by using gene expression and immunohistochemistry studies., Results: All patients had at least a 50% reduction in SCORAD index scores with NB-UVB phototherapy. The T(H)2, T22, and T(H)1 immune pathways were suppressed, and measures of epidermal hyperplasia and differentiation normalized. The reversal of disease activity was associated with elimination of inflammatory leukocytes and T(H)2/T22- associated cytokines and chemokines and normalized expression of barrier proteins., Conclusions: Our study shows that resolution of clinical disease in patients with chronic AD is accompanied by reversal of both the epidermal defects and the underlying immune activation. We have defined a set of biomarkers of disease response that associate resolved T(H)2 and T22 inflammation in patients with chronic AD with reversal of barrier pathology. By showing reversal of the AD epidermal phenotype with a broad immune-targeted therapy, our data argue against a fixed genetic phenotype., (Copyright © 2011 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.)
- Published
- 2011
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10. Cysteinyl leukotriene receptor 2 gene polymorphism -1220 A/C is not associated with atopic dermatitis or psoriasis vulgaris in Japanese patients.
- Author
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Kato T, Saeki H, Tsunemi Y, Shibata S, Sekiya T, Nakamura K, Kakinuma T, Kagami S, Fujita H, Tada Y, Sugaya M, and Tamaki K
- Subjects
- Adult, Case-Control Studies, Dermatitis, Atopic ethnology, Genetic Predisposition to Disease ethnology, Humans, Japan ethnology, Psoriasis ethnology, Dermatitis, Atopic genetics, Genetic Predisposition to Disease genetics, Polymorphism, Single Nucleotide, Psoriasis genetics, Receptors, Leukotriene genetics
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- 2011
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11. Interferon-18 gene polymorphism -137 G/C is associated with susceptibility to psoriasis vulgaris but not with atopic dermatitis in Japanese patients.
- Author
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Kato T, Tsunemi Y, Saeki H, Shibata S, Sekiya T, Nakamura K, Kakinuma T, Kagami S, Fujita H, Tada Y, Sugaya M, and Tamaki K
- Subjects
- Adult, Case-Control Studies, Dermatitis, Atopic ethnology, Gene Frequency, Genetic Predisposition to Disease, Humans, Japan, Middle Aged, Promoter Regions, Genetic, Psoriasis ethnology, Asian People genetics, Dermatitis, Atopic genetics, Interleukin-18 genetics, Polymorphism, Single Nucleotide, Psoriasis genetics
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- 2009
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12. IL-17F single nucleotide polymorphism is not associated with psoriasis vulgaris or atopic dermatitis in the Japanese population.
- Author
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Shibata S, Saeki H, Tsunemi Y, Kato T, Nakamura K, Kakinuma T, Kagami S, Fujita H, Tada Y, Sugaya M, and Tamaki K
- Subjects
- Adult, Aged, Case-Control Studies, Dermatitis, Atopic ethnology, Dermatitis, Atopic immunology, Eosinophils immunology, Female, Gene Frequency, Genetic Predisposition to Disease, Humans, Immunoglobulin E blood, Japan, Leukocyte Count, Male, Middle Aged, Phenotype, Psoriasis ethnology, Psoriasis immunology, Young Adult, Asian People genetics, Dermatitis, Atopic genetics, Interleukin-17 genetics, Polymorphism, Single Nucleotide, Psoriasis genetics
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- 2009
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13. Prevalence of atopic dermatitis determined by clinical examination in Japanese adults.
- Author
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Saeki H, Tsunemi Y, Fujita H, Kagami S, Sasaki K, Ohmatsu H, Watanabe A, and Tamaki K
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- Adult, Age Distribution, Aged, Asian People genetics, Dermatitis, Atopic etiology, Dermatitis, Atopic genetics, Dermatitis, Atopic pathology, Female, Humans, Japan epidemiology, Male, Middle Aged, Physical Examination, Prevalence, Severity of Illness Index, Dermatitis, Atopic epidemiology
- Published
- 2006
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14. Increased serum CCL28 levels in patients with atopic dermatitis, psoriasis vulgaris and bullous pemphigoid.
- Author
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Kagami S, Kakinuma T, Saeki H, Tsunemi Y, Fujita H, Sasaki K, Nakamura K, Takekoshi T, Kishimoto M, Mitsui H, Komine M, Asahina A, and Tamaki K
- Subjects
- Adult, Chemokines, CC, Humans, Chemokines blood, Dermatitis, Atopic immunology, Pemphigoid, Bullous immunology, Psoriasis immunology
- Published
- 2005
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15. The -431C>T polymorphism of thymus and activation-regulated chemokine increases the promoter activity but is not associated with susceptibility to atopic dermatitis in Japanese patients.
- Author
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Tsunemi Y, Komine M, Sekiya T, Saeki H, Nakamura K, Hirai K, Kakinuma T, Kagami S, Fujita H, Asano N, Tanida Y, Wakugawa M, Torii H, and Tamaki K
- Subjects
- Adolescent, Adult, Alleles, Chemokine CCL17, Child, Female, Gene Frequency, Genotype, Humans, Japan, Luciferases metabolism, Male, Middle Aged, Polymorphism, Genetic, Promoter Regions, Genetic, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Transfection, Chemokines, CC genetics, Chemokines, CC physiology, Dermatitis, Atopic genetics, Genetic Predisposition to Disease, Polymorphism, Single Nucleotide
- Abstract
Background: Thymus and activation-regulated chemokine (TARC) plays an important role in the pathogenesis of atopic dermatitis (AD). We recently detected the single nucleotide polymorphism (SNP) (-431C>T) in the 5'-flanking region of TARC gene., Objectives: To examine whether the -431C>T SNP of the TARC gene is associated with susceptibility to AD and whether it affects the promoter activity of the TARC gene., Methods: We investigated the genotype and allele frequencies of the SNP in 193 AD patients and 158 healthy controls by polymerase chain reaction-restriction fragment length polymorphism method. We compared the promoter activities between TARC promoter carrying 431C and that carrying -431T by transient-transfection assay in DJM-1 cell line., Results: There were no significant differences in genotype or allele frequencies between AD patients and controls (genotype: P = 0.38, allele: P = 0.22). Luciferase activity was higher in -431T constructs than in -431C constructs (2.3-fold, P = 9.5 x 10(-6))., Conclusion: These results suggest that the -431C>T SNP of the TARC gene enhances the promoter activity of TARC gene but is not associated with susceptibility to AD in Japanese population.
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- 2004
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16. Lack of association of CCR4 single nucleotide polymorphism with atopic dermatitis in Japanese patients.
- Author
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Tsunemi Y, Sekiya T, Saeki H, Hirai K, Ohta K, Nakamura K, Kakinuma T, Fujita H, Kagami S, Asano N, Tanida Y, Wakugawa M, Torii H, and Tamaki K
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Case-Control Studies, Child, Dermatitis, Atopic ethnology, Eosinophils physiology, Female, Gene Frequency, Genetic Predisposition to Disease, Genotype, Humans, Immunoglobulin E blood, Japan, Leukocyte Count, Male, Middle Aged, Polymerase Chain Reaction, Receptors, CCR4, Asian People genetics, Dermatitis, Atopic genetics, Polymorphism, Single Nucleotide, Receptors, Chemokine genetics
- Abstract
CCR4, a member of the CC chemokine receptor family, is believed to play an important role in the pathogenesis of atopic dermatitis. To examine whether CCR4 single nucleotide polymorphism (SNP) is associated with susceptibility to atopic dermatitis, we investigated the allele and genotype frequencies of C1014T SNP of CCR4 in 198 Japanese patients with atopic dermatitis and controls by a PCR-restriction fragment length polymorphism method. There was no significant difference in allele or genotype frequencies between patients with atopic dermatitis and controls. Serum IgE levels and peripheral blood eosinophil counts were not significantly different among genotypes. There was also no significant difference in allele or genotype frequencies between the patient subgroup with and without asthma, with mild or moderate disease, with and without family history of atopic dermatitis, or with and without family history of atopic disorders. C1014T SNP of CCR4 does not appear to be associated with susceptibility to atopic dermatitis in Japanese patients.
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- 2004
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17. Lack of association of CCR3 single nucleotide polymorphism with atopic dermatitis in Japanese population.
- Author
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Tsunemi Y, Sekiya T, Saeki H, Hirai K, Ohta K, Nakamura K, Kakinuma T, Fujita H, Asano N, Wakugawa M, Torii H, and Tamaki K
- Subjects
- Asian People, Dermatitis, Atopic immunology, Genetic Variation genetics, Humans, Japan, Receptors, CCR3, Dermatitis, Atopic genetics, Polymorphism, Single Nucleotide genetics, Receptors, Chemokine genetics
- Published
- 2003
- Full Text
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18. Increased serum cutaneous T cell-attracting chemokine (CCL27) levels in patients with atopic dermatitis and psoriasis vulgaris.
- Author
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Kakinuma T, Saeki H, Tsunemi Y, Fujita H, Asano N, Mitsui H, Tada Y, Wakugawa M, Watanabe T, Torii H, Komine M, Asahina A, Nakamura K, and Tamaki K
- Subjects
- Adult, Chemokine CCL27, Chemokine CXCL10, Chemokines, CXC blood, Dermatitis, Atopic pathology, Dermatitis, Atopic physiopathology, Humans, Immunohistochemistry methods, Middle Aged, Psoriasis pathology, Psoriasis physiopathology, Severity of Illness Index, Staining and Labeling, Chemokines, CC blood, Dermatitis, Atopic blood, Psoriasis blood
- Abstract
Background: Both atopic dermatitis (AD) and psoriasis vulgaris (PsV) are characterized as chronic and relapsing inflammatory skin diseases associated with various immunologic abnormalities. Cutaneous T cell-attracting chemokine (CTACK; CCL27) is a member of the CC chemokine family and a functional ligand for CC chemokine receptor 10. It is selectively expressed in skin and attracts CC chemokine receptor 10-expressing skin-homing memory T cells. The epidermal keratinocyte is a main source of CTACK, suggesting the involvement of various inflammatory skin diseases., Objective: The purpose of this investigation was to clarify whether CTACK produced by keratinocytes is detected in the sera of patients with AD and PsV and to examine the correlation between the serum CTACK levels and disease activity of patients with AD and PsV., Methods: We measured the serum CTACK levels in 50 patients with AD, 30 patients with PsV, and 22 healthy control subjects. We also divided 50 patients with AD into 3 groups (ie, those with mild, moderate, and severe disease) and compared them among 3 categories. Moreover, we compared the serum CTACK levels of patients with AD and PsV with clinical or laboratory data. Immunohistochemical staining of CTACK and IFN-induced protein of 10 kd (IP-10; CXCL10) was performed on the lesional skin of patients with AD and PsV., Results: The serum CTACK levels in patients with AD and PsV were significantly higher than those in healthy control subjects. The serum CTACK levels in patients with AD significantly correlated with scoring atopic dermatitis (SCORAD) scores, serum soluble IL-2 receptor levels, serum soluble E-selectin levels, serum thymus and activation-regulated chemokine levels, and serum macrophage-derived chemokine levels. Serum CTACK levels in patients with PsV significantly correlated with the serum IP-10 levels but not with the Psoriasis Area and Severity Index score. Immunohistochemical staining showed CTACK was strongly expressed in lesional ke-ratinocytes of patients with AD and PsV, whereas IP-10 was strongly expressed in lesional keratinocytes of patients with PsV and focally in those with AD., Conclusion: These results suggest that CTACK might be one of the important chemokines for the pathogenesis of AD and PsV.
- Published
- 2003
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19. Interleukin-13 gene polymorphism G4257A is associated with atopic dermatitis in Japanese patients.
- Author
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Tsunemi Y, Saeki H, Nakamura K, Sekiya T, Hirai K, Kakinuma T, Fujita H, Asano N, Tanida Y, Wakugawa M, Torii H, and Tamaki K
- Subjects
- Adolescent, Adult, Alleles, Child, Female, Gene Frequency, Genotype, Humans, Japan, Male, Middle Aged, Asian People genetics, Dermatitis, Atopic genetics, Interleukin-13 genetics, Polymorphism, Single Nucleotide
- Abstract
Interleukin (IL)-13 plays an important role in the induction of immunoglobulin E (IgE) and in the pathogenesis of atopic dermatitis (AD). We investigated the allele and genotype frequencies of three IL-13 single nucleotide polymorphisms (SNPs) (A704C and C1103T in the promoter region and G4257A in exon 4) in Japanese patients with AD. For A704C and C1103T SNPs, there were no significant differences in allele or genotype frequencies between AD patients and controls. For G4257A SNP, A allele was significantly increased in AD patients (39.5%) compared with controls (29.4%) (P = 0.016). The same proportion of each genotype and allele was observed in the patient subgroup with and without asthma. Serum IgE levels and peripheral eosinophil counts were not significantly different among genotypes in G4257A SNP. There was also no significant difference in allele or genotype frequencies between AD patients with mild disease and those with severe disease, between those with family history of AD and those without it, or between those with family history of atopic disorders and those without it. This result suggests that 4257A allele is associated with susceptibility to AD and that it may function in the pathogenesis of AD itself, presumably by other mechanisms than inducing IgE production., (Copyright 2002 Elsevier Science Ireland Ltd.)
- Published
- 2002
- Full Text
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20. Interleukin-12 p40 gene (IL12B) 3'-untranslated region polymorphism is associated with susceptibility to atopic dermatitis and psoriasis vulgaris.
- Author
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Tsunemi Y, Saeki H, Nakamura K, Sekiya T, Hirai K, Fujita H, Asano N, Kishimoto M, Tanida Y, Kakinuma T, Mitsui H, Tada Y, Wakugawa M, Torii H, Komine M, Asahina A, and Tamaki K
- Subjects
- Alleles, Gene Frequency, Genotype, Humans, Interleukin-12 Subunit p40, Molecular Sequence Data, 3' Untranslated Regions genetics, Dermatitis, Atopic genetics, Genetic Predisposition to Disease genetics, Interleukin-12 genetics, Polymorphism, Single Nucleotide, Psoriasis genetics
- Abstract
Interleukin-12 (IL-12) is believed to play an important role in inducing Th1-type cytokine profiles. Atopic dermatitis (AD) and psoriasis vulgaris (PsV) are considered to be Th2 and Th1 type disease, respectively. The IL-12 p40 subunit gene (IL12B) is located at chromosome 5q31-33 and linkage findings of AD on 5q31 were reported. Recently single nucleotide polymorphism (SNP) (1188A/C) of IL12B has been reported. In function, it has been reported that this SNP is associated with IL12B mRNA expression levels. To learn whether this SNP is associated with susceptibility to AD or PsV, we investigated the genotype and allele frequencies of the SNP in AD patients, in PsV patients and in controls, examining 164 AD patients, 143 PsV patients and 100 healthy individuals in Japanese population. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method. The A allele was decreased in AD patients (40.9%, p = 0.031) and increased in PsV patients (60.1%, p = 0.035) compared with controls (50.5%). This suggests that IL12B SNP is associated with susceptibility to AD and PsV, presumably by affecting the Th1/Th2 balance., (Copyright 2002 Elsevier Science Ireland Ltd.)
- Published
- 2002
- Full Text
- View/download PDF
21. Eotaxin gene single nucleotide polymorphisms in the promoter and exon regions are not associated with susceptibility to atopic dermatitis, but two of them in the promoter region are associated with serum IgE levels in patients with atopic dermatitis.
- Author
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Tsunemi Y, Saeki H, Nakamura K, Sekiya T, Hirai K, Fujita H, Asano N, Tanida Y, Kakinuma T, Wakugawa M, Torii H, and Tamaki K
- Subjects
- Adolescent, Adult, Chemokine CCL11, Child, Exons genetics, Female, Humans, Male, Middle Aged, Promoter Regions, Genetic genetics, Chemokines, CC genetics, Dermatitis, Atopic blood, Dermatitis, Atopic genetics, Genetic Predisposition to Disease, Immunoglobulin E blood, Polymorphism, Single Nucleotide
- Abstract
Eotaxin is believed to play an important role in atopic dermatitis (AD) as a potent chemoattractant and activator of eosinophils and Th2 lymphocytes. The eotaxin gene is located at chromosome 17q21.1-q21.2, and linkage findings of AD on chromosome 17 were reported. Recently we have identified single nucleotide polymorphisms (SNPs) of eotaxin gene (-426C > T, -384A > G, 67G > A). To learn whether eotaxin gene SNPs are associated with susceptibility to AD or phenotypes of AD, we investigated the genotype frequencies at each SNP of the gene in AD patients and in controls. We examined 140 Japanese AD patients and 140 healthy Japanese individuals. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method. No significant difference was observed in allele or genotype frequencies of any SNP between AD patients and controls. Serum immunoglobulin E (IgE) levels were significantly lower in CT and TT genotype than in CC (P = 0.038) in -426C > T SNP, and lower in GG than in AA and AG with borderline significance (P = 0.053) in -384A > G SNP in AD patients. Eotaxin gene SNPs in the promoter and exon regions are not associated with susceptibility to AD, but two of them in the promoter region are associated with phenotype of AD.
- Published
- 2002
- Full Text
- View/download PDF
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