1. Correlation between miR-148 Expression in Vitreous and Severity of Rhegmatogenous Retinal Detachment.
- Author
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Tsunekawa, Taichi, Kaneko, Hiroki, Takayama, Kei, Hwang, Shiang-Jyi, Oishi, Akio, Nagasaka, Yosuke, Ye, Fuxiang, Iwase, Takeshi, Nonobe, Norie, Ueno, Shinji, Ito, Yasuki, Yasuda, Shunsuke, Matsuura, Toshiyuki, Shimizu, Hideyuki, Suzumura, Ayana, Kataoka, Keiko, and Terasaki, Hiroko
- Subjects
RNA analysis ,OPHTHALMIC surgery ,HEMORRHAGE ,OPHTHALMOSCOPY ,POLYMERASE chain reaction ,PROBABILITY theory ,REGRESSION analysis ,RESEARCH funding ,RETINAL diseases ,RETINAL detachment ,RNA ,STATISTICS ,VITREOUS body ,DATA analysis ,MULTIPLE regression analysis ,SEVERITY of illness index ,DISEASE duration ,DESCRIPTIVE statistics ,MANN Whitney U Test ,DISEASE complications ,SURGERY - Abstract
Purpose. We had earlier reported positive hsa-miR-148a-3p expression in eyes with rhegmatogenous retinal detachment (RRD) and its involvement in the epithelial-mesenchymal transition of retinal pigment epithelium in vitro. Here we investigated the association of hsa-miR-148a-3p expression levels in the vitreous fluid of patients with RRD with severity of RRD. Methods. The hsa-miR-148a-3p expression levels in the vitreous fluid, range (degree) of retinal detachment (RD), and pixels of retinal break were measured in 27 eyes with RRD. The association of hsa-miR-148a-3p expression levels with other factors was evaluated by multiple regression analysis. Results. The hsa-miR-148a-3p expression levels, time from onset of RRD to vitrectomy, range of RD, and pixels of retinal breaks were 23.68±43.00, 12.07±15.36 days, 155.85±86.67 degrees, and 37000±67100 pixels, respectively. Five eyes with RRD had vitreous hemorrhage preoperatively. The hsa-miR-148a-3p expression levels were significantly associated with pixels of retinal breaks (β=0.699) and the time from onset of RRD to vitrectomy (β=0.358) but not with the range of RD or presence of vitreous hemorrhage. Conclusion. The hsa-miR-148a-3p expression levels in the vitreous fluid were significantly associated with the size of retinal break and disease duration. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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