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[Display omitted] • A simple naphthalimide-based chemosensor was synthesized based on ICT process for ratiometric detection of SO 2 derivatives. • The probe displayed high selective and sensitive for derivatives (detection limit is 1.53 μM). • The probe was applied for exogenous and endogenous SO 2 imaging by dual emission channels in living Hela and HepG2 cells. SO 2 is a well-known signal molecule and one of reactive sulfur species, which closely participates in many metabolic processes. While unbalanced metabolism of sulfur dioxide can lead to serious complications of various diseases. Therefore, a rapid and accurate monitoring of SO 2 derivatives with high selectivity and sensitivity would be beneficial for their bio-analytic studies. Herein, a novel ratiometric fluorescent probe (NG-TCF) based on ICT mechanism for monitoring SO 2 was developed. The probe underwent a nucleophilic addition of HSO 3 -/SO 3 2- to give rise to a 120 nm blue-shift dual-emission signal changes in enhanced green channel and subdued red channel under a single wavelength excitation. The probe showed fast response rate (within 7 min), good sensitivity (the detection limit is 1.53 µM), and specific response toward HSO 3 -/SO 3 2- over other bio-species, including H 2 S and ClO-. Moreover, the probe can be applied for visual ratio imaging of exogenous and endogenous SO 2 derivatives in living cells. [ABSTRACT FROM AUTHOR]

[Display omitted] • A simple flavone-based chemosensor was synthesized based on ESIPT process for ratiometric detection of hydrogen polysulfides. • The probe displayed high selective and sensitive for hydrogen polysulfides (detection limit is 0.63 μM). • The probe was successfully applied to exogenous H 2 S n detection and imaging by dual emission channels in living Hela cells. Hydrogen polysulfides (H 2 S n) as an important member of reactive sulfur species is closely relevant to many physiological functions in redox homeostasis and metabolism. Dual-channel monitor the changes of H 2 S n level in vivo is highly desired. Herein we design a simple ratiometric fluorescent probe based on flavone skeleton for highly selective detection of H 2 S n. The probe HF-NA-MC bearing 2-fluoro-5-nitrobenzoic acid group inhibited the intramolecular ESIPT process, which show the blue fluorescence of adjacent naphthalene unit. In the presence of H 2 S n , the enol form of probe is converted to conjugated keto form, resulted in a 90 nm red-shift of fluorescence emission from 450 nm to 540 nm. The ratiometric intensity (I 540 /I 450) of the probe exhibits a good linear relationship toward H 2 S n in the range of 0–120 μM, and the detection limit is estimated to be 0.63 μM. The ratiometric fluorescent probe shows high specificity and anti-interference ability for H 2 S n over other related reactive sulfur species. The probe HF-NA-MC shows promising outlook and could be applied to the confocal imaging of H 2 S n by dual emission channels in Hela cells. [ABSTRACT FROM AUTHOR]