Search

Your search keyword '"Dias, Ana C."' showing total 3 results
Start Over Author "Dias, Ana C." Topic diagnosis
3 results on '"Dias, Ana C."'

1. International Journal of Molecular Sciences / Leishmania infantum -Tubulin Identified by Reverse Engineering Technology through Phage Display Applied as Theranostic Marker for Human Visceral Leishmaniasis

Author

Costa, Lourena E., Alves, Patrícia T., Carneiro, Ana Paula, Dias, Ana C. S., Fujimura, Patrícia T., Araujo, Galber R., Tavares, Grasiele S. V., Ramos, Fernanda F., Duarte, Mariana C., Menezes-Souza, Daniel, Briza, Peter, Briza, Fátima F., and Coelho, Eduardo A. F.

Subjects
diagnosis, tubulin protein, vaccine, phage display, Leishmania infantum, scFv antibody
Abstract

Two Leishmania infantum mimotopes (B10 and C01) identified by phage display showed to be antigenic and immunogenic for visceral (VL) and tegumentary (TL) leishmaniasis; however, their biological targets in the parasites have not been identified. The aim of the present study was to investigate the native antigens expressing both mimotopes, and to use them in distinct immunological assays. For this, a subtractive phage display technology was used, where a combinatorial library of single-chain variable fragments (scFv) was employed and the most reactive monoclonal antibodies for each target were captured, being the target antigens identified by mass spectrometry. Results in immunoblotting and immunoprecipitation assays showed that both monoclonal scFvs antibodies identified the -tubulin protein as the target antigen in L. infantum. To validate these findings, the recombinant protein was cloned, purified and tested for the serodiagnosis of human leishmaniasis, and its immunogenicity was evaluated in PBMC derived from healthy subjects and treated or untreated VL patients. Results showed high diagnostic efficacy, as well as the development of a specific Th1 immune response in the cell cultures, since higher IFN- and lower IL-10 production was found. (VLID)3614782

Published
2019

2. Diagnostic application of sensitive and specific phage-exposed epitopes for visceral leishmaniasis and human immunodeficiency virus coinfection.

Author

Ramos, Fernanda F., Tavares, Grasiele S. V., Ludolf, Fernanda, Machado, Amanda S., Santos, Thaís T. O., Gonçalves, Isabela A. P., Dias, Ana C. S., Alves, Patrícia T., Fraga, Vanessa G., Bandeira, Raquel S., Oliveira-da-Silva, João A., Reis, Thiago A. R., Lage, Daniela P., Martins, Vívian T., Freitas, Camila S., Chaves, Ana T., Guimarães, Nathalia S., Chávez-Fumagalli, Miguel A., Tupinambás, Unaí, and Rocha, Manoel O. C.

Subjects
VISCERAL leishmaniasis, MIXED infections, EPITOPES, HIV, LEISHMANIASIS, ENZYME-linked immunosorbent assay, SENSITIVITY & specificity (Statistics)
Abstract

The diagnosis of visceral leishmaniasis (VL) has improved with the search of novel antigens; however, their performance is limited when samples from VL/human immunodeficiency virus (HIV)-coinfected patients are tested. In this context, studies conducted to identify more suitable antigens to detect both VL and VL/HIC coinfection cases should be performed. In the current study, phage display was performed using serum samples from healthy subjects and VL, HIV-infected and VL/HIV-coinfected patients; aiming to identify novel phage-exposed epitopes to be evaluated with this diagnostic purpose. Nine non-repetitive and valid sequences were identified, synthetized and tested as peptides in enzyme-linked immunosorbent assay experiments. Results showed that three (Pep2, Pep3 and Pep4) peptides showed excellent performance to diagnose VL and VL/HIV coinfection, with 100% sensitivity and specificity values. The other peptides showed sensitivity varying from 50.9 to 80.0%, as well as specificity ranging from 60.0 to 95.6%. Pep2, Pep3 and Pep4 also showed a potential prognostic effect, since specific serological reactivity was significantly decreased after patient treatment. Bioinformatics assays indicated that Leishmania trypanothione reductase protein was predicted to contain these three conformational epitopes. In conclusion, data suggest that Pep2, Pep3 and Pep4 could be tested for the diagnosis of VL and VL/HIV coinfection. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

3. Leishmania infantum β-Tubulin Identified by Reverse Engineering Technology through Phage Display Applied as Theranostic Marker for Human Visceral Leishmaniasis.

Author

Costa, Lourena E., Alves, Patrícia T., Carneiro, Ana Paula, Dias, Ana C. S., Fujimura, Patrícia T., Araujo, Galber R., Tavares, Grasiele S. V., Ramos, Fernanda F., Duarte, Mariana C., Menezes-Souza, Daniel, Briza, Peter, Briza, Fátima F., Coelho, Eduardo A. F., and Goulart, Luiz Ricardo

Subjects
LEISHMANIA infantum, LEISHMANIASIS, IMMUNOPRECIPITATION, IMMUNOBLOTTING, SERODIAGNOSIS
Abstract

Two Leishmania infantum mimotopes (B10 and C01) identified by phage display showed to be antigenic and immunogenic for visceral (VL) and tegumentary (TL) leishmaniasis; however, their biological targets in the parasites have not been identified. The aim of the present study was to investigate the native antigens expressing both mimotopes, and to use them in distinct immunological assays. For this, a subtractive phage display technology was used, where a combinatorial library of single-chain variable fragments (scFv) was employed and the most reactive monoclonal antibodies for each target were captured, being the target antigens identified by mass spectrometry. Results in immunoblotting and immunoprecipitation assays showed that both monoclonal scFvs antibodies identified the β-tubulin protein as the target antigen in L. infantum. To validate these findings, the recombinant protein was cloned, purified and tested for the serodiagnosis of human leishmaniasis, and its immunogenicity was evaluated in PBMC derived from healthy subjects and treated or untreated VL patients. Results showed high diagnostic efficacy, as well as the development of a specific Th1 immune response in the cell cultures, since higher IFN-γ and lower IL-10 production was found. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results