Search

Your search keyword '"Bloodworth S"' showing total 1 results
Start Over Author "Bloodworth S" Topic diamines

Refine your results

1 results on '"Bloodworth S"'

1. Design of maleimide-functionalised electrodes for covalent attachment of proteins through free surface cysteine groups.

Author

Wright EJ, Sosna M, Bloodworth S, Kilburn JD, and Bartlett PN

Subjects
Acetamides chemistry, Biosensing Techniques, Electrochemistry, Electrodes, Molecular Structure, Oxidation-Reduction, Surface Properties, Cysteine chemistry, Cytochromes c chemistry, Diamines chemistry, Maleimides chemical synthesis, Maleimides chemistry, Proteins chemistry
Abstract

Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (≈7.5 pmol cm(-2) )., (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results