1. Dimethylsulfoxide induces upregulation of tumor suppressor protein PTEN through nuclear factor-kappaB activation in HL-60 cells.
- Author
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Lee YR, Shim HJ, Yu HN, Song EK, Park J, Kwon KB, Park JW, Rho HW, Park BH, Han MK, and Kim JS
- Subjects
- Base Sequence, DNA Primers, HL-60 Cells, Humans, Kinetics, PTEN Phosphohydrolase, Phosphoinositide-3 Kinase Inhibitors, Reverse Transcriptase Polymerase Chain Reaction, Dimethyl Sulfoxide pharmacology, Gene Expression Regulation, Neoplastic drug effects, Genes, Tumor Suppressor drug effects, NF-kappa B metabolism, Phosphoric Monoester Hydrolases genetics, Tumor Suppressor Proteins genetics
- Abstract
Dimethylsulfoxide (DMSO) has been known to differentiate HL60 cells into neutrophil like cells. Here, we provide an evidence for the involvement of tumor suppressor PTEN, an antagonist of phosphatidylinositol 3-kinase (PI3K) in the DMSO-induced differentiation of HL60 cells. DMSO upregulated PTEN with unaffecting the expression of PI3K. The upregulation of PTEN by DMSO lead to the decrease of Akt phosphorylation, a downstream of PI3K. The DMSO-induced upregulation of PTEN might be mediated by NF-kappaB activation, which was evidenced by the blockage of DMSO-induced PTEN upregulation with an NF-kappaB inhibitor, pyrrolidine dithiocarbamate (PDTC).
- Published
- 2005
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