1. Prenylated flavins: structures and mechanisms.
- Author
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Bloor S, Michurin I, Titchiner GR, and Leys D
- Subjects
- Flavins metabolism, Flavin Mononucleotide chemistry, Oxidation-Reduction, Carboxy-Lyases genetics, Carboxy-Lyases metabolism, Dimethylallyltranstransferase genetics, Dimethylallyltranstransferase chemistry, Dimethylallyltranstransferase metabolism
- Abstract
The UbiX/UbiD system is widespread in microbes and responsible for the reversible decarboxylation of unsaturated carboxylic acids. The UbiD enzyme catalyzes this unusual reaction using a prenylated flavin (prFMN) as cofactor, the latter formed by the flavin prenyltransferase UbiX. A detailed picture of the biochemistry of flavin prenylation, oxidative maturation, and covalent catalysis underpinning reversible decarboxylation is emerging. This reveals the prFMN cofactor can undergo a wide range of transformations, complemented by considerable UbiD-variability. These provide a blueprint for biotechnological applications aimed at producing hydrocarbons or aromatic C-H activation through carboxylation., (© 2022 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
- Published
- 2023
- Full Text
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