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The growing prevalence of foodborne zoonotic infections increases the risk of emergence of microorganisms with acquired resistance to antimicrobial drugs. Such microorganisms are capable of horizontal transmission of R-plasmids of antibiotic resistance to other types of bacteria. The results of our monitoring in 2021, in accordance with the State Strategy of Ukraine to curb the development of antimicrobial resistance and reduce the risks of the formation and spread of antimicrobial-resistant strains of microorganisms, showed high resistance of enterobacteria, enterococci and Campylobacter isolated from pigs to antibiotics of various groups. Among the isolated cultures of Esherichia coli, the ability of some strains to produce extended-spectrum β-lactamases (ESBL) was found. Testing of the experimental isolates for antibiotic resistance and screening for possible production of acquired resistance enzymes was carried out by the disc diffusion method with antibiotic disks of different groups. Out of 542 samples of appendix from pigs from farms in different regions of Ukraine, 138 isolates were isolated and identified. Among them: Esherichia coli – 68 strains; Enterococcus faecalis – 57; Enterococcus faecium – 9; Salmonella spp. – 2 and Campylobacter spp. – 2. Antibiotic resistance testing showed that 10 (7.3% of the isolates) of different bacterial species remained susceptibile to all antibiotics used. The remaining 128 test isolates (93.5% of the isolates) showed antibiotic resistance. The largest number of antibiotic-resistant commensal and zoonotic bacteria was detected in pig farms of Donetsk, Kyiv, Dnipro, Kirovohrad and Chernihiv regions. Escherichia coli and Enterococcus spp. were most often isolated from pigs; in smaller quantities Salmonella spp. and Campylobacter spp. All strains of Salmonella spp. faecium showed resistance to 1 to 3 antibiotics of different groups. Among the antibiotic-resistant E. faecalis, 1 strain with resistance to vancomycin was detected. Polyantibiotic resistance was inherent in E. colistrains. Among the polyantibiotic-resistant E. colistrains, 4 strains were found and confirmed to have acquired resistance due to the production of ESBL. [ABSTRACT FROM AUTHOR]