1. Analysis of Killer Cell Immunoglobulin-Like Receptor Genes and Their HLA Ligands in Inflammatory Bowel Diseases.
- Author
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Beigmohammadi F, Mahmoudi M, Karami J, Ahmadzadeh N, Ebrahimi-Daryani N, and Rezaei N
- Subjects
- Adult, Alleles, Female, Genetic Predisposition to Disease, Genotype, HLA Antigens genetics, Humans, Inflammatory Bowel Diseases pathology, Killer Cells, Natural immunology, Killer Cells, Natural metabolism, Ligands, Male, Middle Aged, Odds Ratio, Protein Binding, Receptors, KIR metabolism, Young Adult, Disease Susceptibility, HLA Antigens immunology, Inflammatory Bowel Diseases genetics, Inflammatory Bowel Diseases metabolism, Receptors, KIR genetics
- Abstract
Genetic studies have illustrated that killer cell immunoglobulin-like receptor ( KIR ) genes could participate in various autoimmune disorders. We aimed to clarify the role of KIR genes, HLA ligands, HLA-KIR interactions, and their genotypes in inflammatory bowel disease (IBD) susceptibility. The study population was composed of 183 IBD subjects, comprising 100 ulcerative colitis (UC) patients, 83 Crohn's disease (CD) patients, and 274 healthy subjects. Polymerase chain reaction with sequence-specific primers (PCR-SSP) was used to evaluate the absence or presence of the 15 KIR genes, 5 HLA class I ligands, and 2 pseudogenes. We did not find any significant difference in allele frequency of KIRs and pseudogenes between IBD patients and healthy controls. In the case of HLA genes, there was a significant difference in HLA-B-Bw4
Thr80 frequency between UC patients and healthy controls ( P = 0.03, OR = 0.06, 95%CI = 0.008-0.4). Furthermore, we found a significant difference in HLA-C1Asn80 frequency between CD patients and healthy controls ( P = 0.04, OR = 0.49, 95% CI = 0.3-0.8). In the full-array combination of KIR genes, there was no significant frequency difference between UC patients and healthy controls, while two KIR genotypes showed a significant susceptible association with CD. Our data do not support a strong role of NK cells in IBD susceptibility, but it does not rule out a role for KIR variability in IBD patients. However, there are some protective associations such as Bw4 alleles; these associations may be due to the interaction of the alleles to TCRs rather than KIRs., Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper., (Copyright © 2020 Fereshteh Beigmohammadi et al.)- Published
- 2020
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