1. Four diterpenes identified in silico were isolated from Hyptidinae and demonstrated in vitro activity against Mycobacterium tuberculosis .
- Author
-
Cavalcanti ABS, Maia MDS, Figueiredo PTR, Menezes RPB, Monteiro AFM, Meireles RAR, Rodrigues GCS, Rodrigues de Almeida Silva AR, Lins JDS, Cordeiro LV, Junior VSR, Castelo Branco APOT, Agra MF, Sessions ZL, Muratov EN, Scotti L, Silva MSD, Costa VCO, Tavares JF, and Scotti MT
- Subjects
- Microbial Sensitivity Tests, Antitubercular Agents chemistry, Mycobacterium tuberculosis, Diterpenes chemistry, Tuberculosis, Lamiaceae chemistry
- Abstract
Plants of Hyptidinae subtribe (Lamiaceae - family), as Mesosphaerum sidifolium , are a source of bioactive molecules. In the search for new drug candidates, we perform chemical characterization of diterpenes isolated from the aerial parts of M. sidifolium was carried out with uni- and bidimensional NMR spectral data, and evaluate in silico through the construction of a predictive model followed by in vitro testing Mycobacterium tuberculosis and Mycobacterium smegmatis . Resulted in the isolation of four components: Pomiferin D (1), Salviol (2), Pomiferin E (3) and 2α-hydroxysugiol (4), as well as two phenolic compounds, rosmarinic and caffeic acids. In silico model identified 48 diterpenes likely to have biological activity against M. tuberculosis . The diterpenes isolated were tested in vitro against M. tuberculosis demonstrating MIC = 125 µM for 4 and 1, while 2 and 3 -MIC = 250 µM. These compounds did not show biological activity at these concentrations for M. smegmatis .
- Published
- 2023
- Full Text
- View/download PDF