1. Population genetics and phylogenetic analysis of the vrs1 nucleotide sequence in wild and cultivated barley.
- Author
-
Ren, Xifeng, Wang, Yonggang, Yan, Songxian, Sun, Dongfa, Sun, Genlou, and Gustafson, J.P.
- Subjects
- *
BARLEY , *PLANT genetics , *NUCLEOTIDE sequence , *HORDEUM , *GENETICS - Abstract
Spike morphology is a key characteristic in the study of barley genetics, breeding, and domestication. Variation at the six-rowed spike 1 ( vrs1) locus is sufficient to control the development and fertility of the lateral spikelet of barley. To study the genetic variation of vrs1 in wild barley ( Hordeum vulgare subsp. spontaneum) and cultivated barley ( Hordeum vulgare subsp. vulgare), nucleotide sequences of vrs1 were examined in 84 wild barleys (including 10 six-rowed) and 20 cultivated barleys (including 10 six-rowed) from four populations. The length of the vrs1 sequence amplified was 1536 bp. A total of 40 haplotypes were identified in the four populations. The highest nucleotide diversity, haplotype diversity, and per-site nucleotide diversity were observed in the Southwest Asian wild barley population. The nucleotide diversity, number of haplotypes, haplotype diversity, and per-site nucleotide diversity in two-rowed barley were higher than those in six-rowed barley. The phylogenetic analysis of the vrs1 sequences partially separated the six-rowed and the two-rowed barley. The six-rowed barleys were divided into four groups. [ABSTRACT FROM AUTHOR] more...
- Published
- 2014
- Full Text
- View/download PDF