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Objective: We assessed whether Petrus Donders (died 1887), a Dutch priest who for 27 years cared for people with leprosy in the leprosarium Batavia, Suriname, had evidence of Mycobacterium (M.) leprae infection. A positive finding of M. leprae ancient (a)DNA would contribute to the origin of leprosy in Suriname., Materials: Skeletal remains of Father Petrus Donders; two additional skeletons excavated from the Batavia cemetery were used as controls., Methods: Archival research, paleopathological evaluation and aDNA-based testing of skeletal remains., Results: Neither archives nor inspection of Donders skeletal remains revealed evidence of leprosy, and aDNA-based testing for M. leprae was negative. We detected M. leprae aDNA by RLEP PCR in one control skeleton, which also displayed pathological lesions compatible with leprosy. The M. leprae aDNA was genotyped by Sanger sequencing as SNP type 4; the skeleton displayed mitochondrial haplogroup L3., Conclusion: We found no evidence that Donders contracted leprosy despite years of intense leprosy contact, but we successfully isolated an archaeological M. leprae aDNA sample from a control skeleton from South America., Significance: We successfully genotyped recovered aDNA to a M. leprae strain that likely originated in West Africa. The detected human mitochondrial haplogroup L3 is also associated with this geographical region. This suggests that slave trade contributed to leprosy in Suriname., Limitations: A limited number of skeletons was examined., Suggestions for Further Research: Broader review of skeletal collections is advised to expand on diversity of the M. leprae aDNA database., (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)